Difference between revisions of "Gabrielova 2011 Abstract IOC61"
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{{Abstract | {{Abstract | ||
|title= | |title=Gabrielova E, Jaburek M, Gazak R, Vostalova J, Jezek J, Kren V, Modriansky M (2011) Evaluation of an oxygen consumption in rat heart mitochondria treatment by polyphenol compounds. MiPNet16.01. | ||
|authors=Gabrielova E, Jaburek M, Gazak R, Vostalova J, Jezek J, Kren V, Modriansky M | |authors=Gabrielova E, Jaburek M, Gazak R, Vostalova J, Jezek J, Kren V, Modriansky M | ||
|year=2011 | |year=2011 | ||
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Silybin and dehydrosilybin, two polyphenolic compounds, display a plethora of biological effects generally ascribed to their known antioxidant capacity. Experiments evaluating oxygen consumption and membrane potential revealed that dehydrosilybin uncouples the respiration of isolated rat heart mitochondria albeit with a much lower potency than synthetic uncouplers. Furthermore, dehydrosilybin revealed a very high potency in suppressing ROS formation in isolated rat heart mitochondria with IC<sub>50</sub>=0.15 μM. | Silybin and dehydrosilybin, two polyphenolic compounds, display a plethora of biological effects generally ascribed to their known antioxidant capacity. Experiments evaluating oxygen consumption and membrane potential revealed that dehydrosilybin uncouples the respiration of isolated rat heart mitochondria albeit with a much lower potency than synthetic uncouplers. Furthermore, dehydrosilybin revealed a very high potency in suppressing ROS formation in isolated rat heart mitochondria with IC<sub>50</sub>=0.15 μM. | ||
We infer that the apparent uncoupler-like activity of dehydrosilybin is the basis of its ROS modulation effect in neonatal rat cardiomyocytes and leads us to propose a hypothesis on natural ischemia preconditioning by dietary polyphenols. | We infer that the apparent uncoupler-like activity of dehydrosilybin is the basis of its ROS modulation effect in neonatal rat cardiomyocytes and leads us to propose a hypothesis on natural ischemia preconditioning by dietary polyphenols. | ||
|mipnetlab=CZ Olomouc Modriansky M | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |organism=Rat | ||
|tissues= | |tissues=Heart | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
| | |injuries=Oxidative stress;RONS | ||
|additional=dehydrosilybin, silybin | |additional=dehydrosilybin, silybin | ||
}} | }} | ||
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Gabrielová E<sup>1</sup>, Jabůrek M<sup>2</sup>, Gažák R<sup>3</sup>, Vostálová J<sup>1</sup>, Ježek J<sup>2</sup>, Křen V<sup>3</sup> and Modrianský M<sup>1</sup> | |||
#Institute of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic | #Institute of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic | ||
#Institute of Physiology, Dept. 75, Academy of Sciences of the Czech Republic, Prague, Czech Republic | #Institute of Physiology, Dept. 75, Academy of Sciences of the Czech Republic, Prague, Czech Republic | ||
#Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic | #Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic | ||
[[Has mailaddress::{{{mailaddress|[email protected]}}}]] | :[[Has mailaddress::{{{mailaddress|[email protected]}}}]] | ||
Latest revision as of 14:24, 13 February 2015
| Gabrielova E, Jaburek M, Gazak R, Vostalova J, Jezek J, Kren V, Modriansky M (2011) Evaluation of an oxygen consumption in rat heart mitochondria treatment by polyphenol compounds. MiPNet16.01. |
Link:
Gabrielova E, Jaburek M, Gazak R, Vostalova J, Jezek J, Kren V, Modriansky M (2011)
Event: IOC61
Reactive oxygen species (ROS) originating from mitochondria are perceived as a factor contributing to cell aging and means have been sought to attenuate ROS formation with the aim of extending the cell lifespan. Silybin and dehydrosilybin, two polyphenolic compounds, display a plethora of biological effects generally ascribed to their known antioxidant capacity. Experiments evaluating oxygen consumption and membrane potential revealed that dehydrosilybin uncouples the respiration of isolated rat heart mitochondria albeit with a much lower potency than synthetic uncouplers. Furthermore, dehydrosilybin revealed a very high potency in suppressing ROS formation in isolated rat heart mitochondria with IC50=0.15 μM. We infer that the apparent uncoupler-like activity of dehydrosilybin is the basis of its ROS modulation effect in neonatal rat cardiomyocytes and leads us to propose a hypothesis on natural ischemia preconditioning by dietary polyphenols.
• O2k-Network Lab: CZ Olomouc Modriansky M
Labels:
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Heart Preparation: Isolated mitochondria
dehydrosilybin, silybin
Gabrielová E1, Jabůrek M2, Gažák R3, Vostálová J1, Ježek J2, Křen V3 and Modrianský M1
- Institute of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic
- Institute of Physiology, Dept. 75, Academy of Sciences of the Czech Republic, Prague, Czech Republic
- Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
