Difference between revisions of "Glucose"
From Bioblast
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::'''Glc: Glucose''' (D-(+)-Glucose, C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at RT; | :::'''Glc: Glucose''' (D-(+)-Glucose, C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at RT; M = 180.16 g·mol<sup>-1</sup> | ||
Revision as of 13:12, 12 February 2021
- high-resolution terminology - matching measurements at high-resolution
Glucose
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
Application in HRR
- Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at RT; M = 180.16 g·mol-1
- Preparation of 2 M stock solution (dissolved in H2O):
- weigh 360.32 mg of Glucose
- dissolve 1 mL H2O in 1.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 20 mM
- Preparation of 2.2 M stock solution (dissolved in H2O):
- weigh 79.2 mg of Glucose
- dissolve 0.2 mL H2O in 0.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 5.5 mM (1 g/L)
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
- Final concentration: 11 mM (2 g/L)
MitoPedia topics: Substrate and metabolite
