Difference between revisions of "Glucose"
From Bioblast
(replaced FW by M and added units g*mol-1) |
(updated preparations instructions to take volume of the solute into account) |
||
| Line 11: | Line 11: | ||
::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | ::::'''Preparation of 2 M stock solution''' (1 mL, dissolved in H<sub>2</sub>O): | ||
::::# weigh 360.32 mg of Glucose | ::::# weigh 360.32 mg of Glucose | ||
::::# dissolve | ::::# dissolve in 775 µL H<sub>2</sub>O in 1.5 mL Eppendorf | ||
:::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
| Line 23: | Line 22: | ||
::::'''Preparation of 2.2 M stock solution''' (dissolved in H<sub>2</sub>O): | ::::'''Preparation of 2.2 M stock solution''' (200 µL, dissolved in H<sub>2</sub>O): | ||
::::# weigh 79.2 mg of Glucose | ::::# weigh 79.2 mg of Glucose | ||
::::# dissolve | ::::# dissolve 155 µL H<sub>2</sub>O in 0.5 mL Eppendorf | ||
:::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
| Line 34: | Line 33: | ||
::::* Titration volume ('''2-mL O2k-chamber'''): 10 µL using a 25 µL Hamilton syringe. | ::::* Titration volume ('''2-mL O2k-chamber'''): 10 µL using a 25 µL Hamilton syringe. | ||
::::* Final concentration: 11 mM (2 g/L) | ::::* Final concentration: 11 mM (2 g/L) | ||
<!-- | <!-- | ||
::::* Titration volume: 5 µL using a 25 µL syringe | ::::* Titration volume: 5 µL using a 25 µL syringe | ||
::::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L) | ::::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L) | ||
| Line 42: | Line 39: | ||
::::* Titration volume: 10 µL using a 25 µL syringe | ::::* Titration volume: 10 µL using a 25 µL syringe | ||
::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) | ::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) | ||
--> | --> | ||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
Revision as of 13:05, 8 March 2021
- high-resolution terminology - matching measurements at high-resolution
Glucose
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
Application in HRR
- Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at RT; M = 180.16 g·mol-1
- Preparation of 2 M stock solution (1 mL, dissolved in H2O):
- weigh 360.32 mg of Glucose
- dissolve in 775 µL H2O in 1.5 mL Eppendorf
- Preparation of 2 M stock solution (1 mL, dissolved in H2O):
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 20 mM
- Preparation of 2.2 M stock solution (200 µL, dissolved in H2O):
- weigh 79.2 mg of Glucose
- dissolve 155 µL H2O in 0.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 5.5 mM (1 g/L)
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
- Final concentration: 11 mM (2 g/L)
MitoPedia topics:
Substrate and metabolite
