Glucose

high-resolution terminology - matching measurements at high-resolution

Glucose

Description

Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.

Abbreviation: Glc

Reference: MiPNet15.09. Yeast: HRR Reference Assay

Application in HRR

Glc: Glucose (D-(+)-Glucose, C₆H₁₂0₆); Sigma G 8270, 100 g, store at RT; M = 180.16 g·mol^-1

2021-03: The preparation instructions were updated to take the volume of the solute into account (see: Volume_of_the_solute). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.

Preparation of 2 M stock solution (1 mL, dissolved in H₂O):

1. weigh 360.32 mg of Glucose
2. dissolve in 775 µL H₂O in 1.5 mL Eppendorf

»O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
• Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
• Final concentration: 20 mM

Preparation of 2.2 M stock solution (200 µL, dissolved in H₂O):

1. weigh 79.2 mg of Glucose
2. dissolve 155 µL H₂O in 0.5 mL Eppendorf

»O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
• Final concentration: 5.5 mM (1 g/L)

• Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
• Final concentration: 11 mM (2 g/L)

MitoPedia topics: Substrate and metabolite