Glucose: Difference between revisions
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|abbr=Glc | |abbr=Glc | ||
|description='''Glucose''', also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate. | |description='''Glucose''', also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate. | ||
|info=[ | |info=[[MiPNet15.09. Yeast: HRR Reference Assay]] | ||
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{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
}} | }} | ||
== Application in [[HRR]]ย == | == Application in [[HRR]]ย == | ||
Revision as of 09:30, 2 June 2016
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
MitoPedia topics: Substrate and metabolite
Application in HRR
Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at R.T.; FW = 180.16
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 360.32 mg of Glucose
- 2) dissolve in 1 ml H2O
Oxygraph-2k Titration:
- Titration volume: 20 ยตl using a 50 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 20 mM