Difference between revisions of "Glucose"
From Bioblast
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::1) weigh 360.32 mg of Glucose ย | ::1) weigh 360.32 mg of Glucose ย | ||
::2) dissolve | ::2) dissolve 1 ml H<sub>2</sub>O in 1,5ml Eppendorf | ||
ย | |||
'''Oxygraph-2k Titration:'''ย ย | '''Oxygraph-2k Titration:'''ย ย |
Revision as of 11:26, 7 September 2016
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
MitoPedia topics: Substrate and metabolite
Application in HRR
Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at R.T.; FW = 180.16
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 360.32 mg of Glucose
- 2) dissolve 1 ml H2O in 1,5ml Eppendorf
Oxygraph-2k Titration:
- Titration volume: 20 ยตl using a 50 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 20 mM