Hecker 2015 Methods Mol Biol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Hecker M, Sommer N, Mayer K (2015) Assessment of short- and medium-chain fatty acids on mitochondrial function in severe | |title=Hecker M, Sommer N, Mayer K (2015) Assessment of short- and medium-chain fatty acids on mitochondrial function in severe Inflammation. Methods Mol Biol 1265:389-96. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/25634290 PMID:25634290] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/25634290 PMID:25634290] | ||
|authors=Hecker M, Sommer N, Mayer K | |authors=Hecker M, Sommer N, Mayer K | ||
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monocytes were pretreated with TNF-ฮฑ to mimic infl ammation followed by incubation with SCFA/MCFA | monocytes were pretreated with TNF-ฮฑ to mimic infl ammation followed by incubation with SCFA/MCFA | ||
and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR. | and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR. | ||
|keywords=Mitochondria, Respiration, Oxygen consumption, SCFA, MCFA, TNF-ฮฑ | |keywords=Mitochondria, Respiration, Oxygen consumption, SCFA, MCFA, TNF-ฮฑ, HUVEC | ||
|mipnetlab=DE Giessen Weissmann N | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics | |area=Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics | ||
|diseases=Sepsis | |||
|organism=Human | |organism=Human | ||
|tissues=Endothelial;epithelial;mesothelial cell | |tissues=Endothelial;epithelial;mesothelial cell | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|topics=Coupling efficiency;uncoupling, Uncoupler, Fatty acid | |topics=Coupling efficiency;uncoupling, Uncoupler, Fatty acid | ||
|couplingstates=LEAK, ROUTINE, | |couplingstates=LEAK, ROUTINE, ET | ||
| | |pathways=ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 14:34, 13 November 2017
| Hecker M, Sommer N, Mayer K (2015) Assessment of short- and medium-chain fatty acids on mitochondrial function in severe Inflammation. Methods Mol Biol 1265:389-96. |
Hecker M, Sommer N, Mayer K (2015) Methods Mol Biol
Abstract: Mitochondrial dysfunction is regarded as one key factor involved in the pathogenesis of septic disorders, leading to a decline in energy supply. The infl uence of short- and medium-chain fatty acids (SCFA/MCFA) on mitochondrial respiration under infl ammatory conditions has thus far not been investigated. In the following protocol, we describe the assessment of mitochondrial respiration using high-resolution respirometry under infl ammatory and baseline conditions. For this approach, human endothelial cells and monocytes were pretreated with TNF-ฮฑ to mimic infl ammation followed by incubation with SCFA/MCFA and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR. โข Keywords: Mitochondria, Respiration, Oxygen consumption, SCFA, MCFA, TNF-ฮฑ, HUVEC
โข O2k-Network Lab: DE Giessen Weissmann N
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics
Pathology: Sepsis
Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Intact cells
Regulation: Coupling efficiency;uncoupling, Uncoupler, Fatty acid Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k
