Difference between revisions of "Hogeboom 1946 J Biol Chem"
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|year=1946 | |year=1946 | ||
|journal=J Biol Chem | |journal=J Biol Chem | ||
|abstract= | |abstract=See Free Text | ||
|keywords=cytochrome oxidase, succinoxidase, liver cell extract | |keywords=cytochrome oxidase, succinoxidase, liver cell extract | ||
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|additional=Made history | |additional=Made history | ||
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SUMMARY | |||
1. A study of the distribution of two enzyme systems, cytochrome oxidase | |||
and succinoxidase, in the cytoplasm of the liver cell of the rat has been | |||
presented. A liver extract, containing only cytoplasmic material and suitable for fractionation by differential centrifugation, was utilized as an experimental tool. | |||
2. Both enzyme systems were shown to be associated, probably exclusively, | |||
with sedimentable granules of a relatively large size (0.5 to 2 p in | |||
diameter). A smaller sedimentable component (60 to 150 rnp in diameter), | |||
which was present in large amount in the liver extract, showed little, if any, | |||
enzyme activity and the remaining soluble material of the extract showed no | |||
activity. A number of observations suggested that the enzymatically active | |||
large granule fraction consisted chiefly of those cytoplasmic inclusions | |||
known as mitochondria. | |||
3. The large granules were markedly affected by changes in tonicity of | |||
the surrounding medium, their disintegration in hypotonic media being | |||
accompanied by a rapid and pronounced loss in aerobic succinoxidase | |||
activity. This decline in succinoxidase could not be explained either | |||
by loss of cytochrome oxidase activity, which remained at a high level, | |||
or, from the results of anaerobic determinations, by loss of succinic dehydrogenase itself. By repeated washing of the large granules in a hypotonic | |||
medium, it was possible, however, to reduce their anaerobic succinic | |||
dehydrogenase activity to a low level, apparently through the release of a | |||
soluble component of the enzyme system. | |||
4. The ingestion over a period of less than 2 months of the carcinogenic | |||
substance, p-dimethylaminoazobenzene, produced a reduction in the | |||
succinoxidase content of the large granules of rat liver. | |||
Revision as of 17:29, 7 June 2012
| Hogeboom GH, Claude A, Hotchkiss RD (1946) The distribution of cytochrome oxidase and succinoxidase in the cytoplasm of the mammalian liver cell. J Biol Chem 165: 615-629. |
Hogeboom GH, Claude A, Hotchkiss RD (1946) J Biol Chem
Abstract: See Free Text • Keywords: cytochrome oxidase, succinoxidase, liver cell extract
Labels:
Organism: Rat
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Homogenate
Enzyme: Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
Made history
SUMMARY 1. A study of the distribution of two enzyme systems, cytochrome oxidase and succinoxidase, in the cytoplasm of the liver cell of the rat has been presented. A liver extract, containing only cytoplasmic material and suitable for fractionation by differential centrifugation, was utilized as an experimental tool. 2. Both enzyme systems were shown to be associated, probably exclusively, with sedimentable granules of a relatively large size (0.5 to 2 p in diameter). A smaller sedimentable component (60 to 150 rnp in diameter), which was present in large amount in the liver extract, showed little, if any, enzyme activity and the remaining soluble material of the extract showed no activity. A number of observations suggested that the enzymatically active large granule fraction consisted chiefly of those cytoplasmic inclusions known as mitochondria. 3. The large granules were markedly affected by changes in tonicity of the surrounding medium, their disintegration in hypotonic media being accompanied by a rapid and pronounced loss in aerobic succinoxidase activity. This decline in succinoxidase could not be explained either by loss of cytochrome oxidase activity, which remained at a high level, or, from the results of anaerobic determinations, by loss of succinic dehydrogenase itself. By repeated washing of the large granules in a hypotonic medium, it was possible, however, to reduce their anaerobic succinic dehydrogenase activity to a low level, apparently through the release of a soluble component of the enzyme system. 4. The ingestion over a period of less than 2 months of the carcinogenic substance, p-dimethylaminoazobenzene, produced a reduction in the succinoxidase content of the large granules of rat liver.
