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| '''O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced'''
| | #REDIRECT [[MiPNet15.10_IOC60]] |
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| Schröcken, Vorarlberg, Austria 11-16 Dec, 2010.
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| More information on IOC60: http://www.oroboros.at/index.php?nextcourse
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| ==Format==
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| Hot Topics in Mitochondrial Physiology: 10+5 min
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| ==Abstracts==
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| *[[:Category:IOC60|Look at Abstracts >>]]
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| For the abstract format, see [[Author_IOC60]].
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| *[[:Form:Publication|Add your Abstract >>]]
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| Important for adding your abstract: <nowiki>please copy/paste [[Category:IOC60]] into the 'Freetext' field</nowiki>
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| == Tutors ==
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| * [[Gnaiger E|Erich Gnaiger]]
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| * [[Fasching M|Mario Fasching ]]
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| * [[Pesta D|Dominik Pesta]]
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| * [[Sumbalova Z|Zuzana Sumbalova]]
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| * [[Wiethuechter A|Anita Wiethuechter]]
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| == Combined introductory and advanced O2k-Workshop with parallel sessions ==
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| A: Introductory lectures and hands-on experiments for basic HRR in small working teams:
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| '''A1. General [[HRR]] topics'''
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| * O2k-assembly,
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| * [[Polarographic oxygen sensor]] (OroboPOS) service,
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| * POS calibration,
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| * [[DatLab]] software: principles and operation,
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| * [[Titration-Injection microPump]] - TIP2k.
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| '''A2. Protocols in HRR'''
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| * Introduction to fundamental elements of mitochondrial respiratory physiology,
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| * Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
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| '''A3. Hands-on experiments with the O2k'''
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| * Measurement of [[instrumental background oxygen flux]], manual and TIP2k-feedback control,
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| * Intact cells: Respirometric reference assay with yeast, [[phosphorylation control protocol]],
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| * Preparation of [[permeabilized muscle fibre]]s,
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| * HRR with permeabilized muscle fibres.
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| '''A4. Presentation and interpretation of [[oxygen flux]]'''
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| * Normalization of respiration per tissue or per [[mitochondrial marker]],
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| * Coupling control ratios,
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| * Substrate control ratios,
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| * Generalized flux control ratios.
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| '''A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:'''
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| * Mitochondrial membrane potential (TPP+ electrode),
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| * Acidification (pH electrode),
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| * Nitric oxide (amperometric),
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| * Spectrophotometry (cytochrome spectra),
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| * Spectrofluorimetry (Amplex red, safranin, etc.)
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| B: Advanced users will join in small working teams in parallel sessions, with specific topics:
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| '''B1. O2k-MultiSensor and TIP2k'''
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| * Hands-on MultiSensor TPP<sup>+</sup> applications,
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| * Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
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| * ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
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| * pH-stat feedback control,
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| '''B2. Theoretical perspectives'''
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| * Advanced experimental protocols,
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| * Quality control - towards [[quality assurance]];
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| * ''Add your topic'':
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| == Participants and areas of interest ==
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| * '''Drabarek Beata, Poland''': Respiration measurements in endothelial cells under hyperglycemic conditions.
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| * [[El-Bacha T|'''El-Bacha Tatiana, Brazil''']]: My research interests are studies on mitochondrial physiology of mammalian cells and animal models of virus infections (alphavirus and flavivirus). Currently, we are investigating the relationship of hepatic function and oxidative stress in mice infected with dengue virus and the possible implications for the severe forms of infection. ''Keywords'': virus infection; dengue; mitochondrial bioenergetics; mice; liver dysfunction; HepG2 cells.
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| * '''Felser Andrea, Switzerland'''
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| * [[Rosenfeld E|'''Rosenfeld Eric, France''']]: Characterization of non-respiratory oxygen consumption pathways in yeast (oxygen and ROS fluxes); study of redox-active molecules targeting eukaryotic or prokaryotic microorganisms (i.e. ''Pseudomonas aeruginosa'' strains involved in cystic fibrosis). ''Keywords'': Yeast, bacteria, respiration, non-respiratory pathways, ROS, redox active molecules.
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| * '''Staples James, Canada''': Suppression of metabolism in hibernation. ''Keywords'': hibernation, torpor, mitochondria.
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| * '''Wojciechowska Sylwia, Poland''': Respiration measurements in different types of cells in insulin resistance state.
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| ==Discussion==
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| [[Talk:IOC60|Continue the Discussion >>]]
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| [[Category:IOC60]]
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| [[Category:O2k-Workshops]]
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| [[Category:Events]]
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