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| '''O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced'''
| | #REDIRECT [[MiPNet16.01 IOC61]] |
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| Schröcken, Vorarlberg, Austria 26 April - 1 May, 2011.
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| Hotel Körbersee
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| For some pictures, see [[IOC60_Photos|IOC60]]
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| More information on IOC61: http://www.oroboros.at/index.php?nextcourse
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| ==Format==
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| * Introductory and advanced groups will be working in parallel session, to adjust the programme optimally to the specific needs of the participants in different workpackages.
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| * Hot Topics in Mitochondrial Physiology: 10+5 min
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| ==Abstracts==
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| *[[:Category:IOC61|Look at Abstracts >>]]
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| For the abstract format, see [[Author_IOC61]].
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| *[[:Form:Publication|Add your Abstract >>]]
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| Important for adding your abstract: <nowiki>please copy/paste [[Category:IOC61]] into the 'Freetext' field</nowiki>
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| == Combined introductory and advanced O2k-Workshop with parallel sessions ==
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| ::'''A: Introductory lectures and hands-on experiments for basic HRR in small working teams:'''
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| '''A1. General [[HRR]] topics'''
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| * O2k-assembly,
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| * [[Polarographic oxygen sensor]] (OroboPOS) service,
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| * POS calibration,
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| * [[DatLab 4.3|DatLab]] software: principles and operation,
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| * [[Titration-Injection-microPump]] - TIP2k.
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| '''A2. Protocols in HRR'''
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| * Introduction to fundamental elements of mitochondrial respiratory physiology,
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| * Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
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| '''A3. Hands-on experiments with the O2k'''
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| * Measurement of [[instrumental background oxygen flux]], manual and TIP2k-feedback control,
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| * Intact cells: Respirometric reference assay with yeast, [[phosphorylation control protocol]],
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| * Preparation of [[permeabilized muscle fibre]]s,
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| * HRR with permeabilized muscle fibres.
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| '''A4. Presentation and interpretation of [[oxygen flux]]'''
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| * Normalization of respiration per tissue or per [[mitochondrial marker]],
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| * Coupling control ratios,
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| * Substrate control ratios,
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| * Generalized flux control ratios.
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| '''A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:'''
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| * Mitochondrial membrane potential (TPP+ electrode),
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| * Acidification (pH electrode),
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| * Nitric oxide (amperometric),
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| * Spectrophotometry (cytochrome spectra),
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| * Spectrofluorimetry (Amplex red, safranin, etc.)
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| ::'''B: Advanced users will join in small working teams in parallel sessions, with specific topics:
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| '''
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| '''B1. O2k-MultiSensor and TIP2k'''
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| * Hands-on MultiSensor TPP<sup>+</sup> applications, see [[TPP special interest group during an International Oxygraph Course]]
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| * Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
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| * ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
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| * pH-stat feedback control,
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| '''B2. Theoretical perspectives'''
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| * Advanced experimental protocols,
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| * Quality control - towards [[quality assurance]];
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| * ''Add your topic'':
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| ==Participants and areas of interest==
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| * [[Hals I|'''Ingrid Hals, Norway''']]: To study effects of hypoxia in pancreatic beta-cells on mitochondrial functions.
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| * [[Sanz A|'''Alberto Sanz, Finland''']]: Mechanism causing aging and the role of mitochondria in this process.
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| * [[Yokota T|'''Takashi Yokota, Denmark''']]: The role of mitochondria in chronic heart failure, diabetes, or obesity.
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| * [[Alexeyev M|'''Mikhail Alexeyev, USA''']]: Mouse modeling of mitochondrial disorders caused by mtDNA mutations.
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| == Tutors ==
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| * [[Gnaiger E|'''Erich Gnaiger''']] (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
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| * [[Fasching M|'''Mario Fasching''']] (OROBOROS INSTRUMENTS, AT)
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| * [[Pesta D|'''Dominik Pesta''']] (Innsbruck Medical University, AT)
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| * [[Sumbalova Z|'''Zuzana Sumbalova''']] (OROBOROS INSTRUMENTS, AT)
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| * [http://www.oroboros.at/index.php?oroboros-contact '''Contact''']: Katrin Stecher, ''administration''; Email [[ Has mailaddress::{{{mailaddress|katrin. [email protected]}}}]] | |
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| ==Discussion==
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| [[Talk:IOC61|Continue the Discussion >>]]
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| [[Category:IOC61]]
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| [[Category:O2k-Workshops]]
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| [[Category:Events]]
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