Jarolim 2004 FEMS Yeast Res: Difference between revisions
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|area=Respiration | |area=Respiration | ||
|organism=Saccharomyces cerevisiae | |organism=Saccharomyces cerevisiae, Fungi | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS |
Revision as of 09:23, 9 November 2016
Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) A novel assay for replicative lifespan in Saccharomyces cerevisiae. FEMS Yeast Res 5:169-77. |
Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) FEMS Yeast Res
Abstract: The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants. The assay reproduces the lifespan-shortening effects of deleting SIR2 and of growth in the presence of paraquat, a pro-oxidant. The lifespan-increasing activity of resveratrol is also reproduced. Compared to current assays, this new strategy promises to significantly increase the possible number of replicative-lifespan determinations. โข Keywords: Saccharomyces cerevisiae, Yeast, Longevity, Aging, Replicative lifespan
โข O2k-Network Lab: AT Salzburg Breitenbach M
Labels: MiParea: Respiration
Organism: Saccharomyces cerevisiae, Fungi
Preparation: Intact cells
Coupling state: OXPHOS
HRR: Oxygraph-2k