Jeger 2015 BioMed Res Internat

Jeger V, Brandt S, Porta F, Jakob SM, Takala J, Djafarzadeh S (2014) Dose response of rndotoxin on hepatocyte and muscle mitochondrial respiration In vitro. Hindawi Pub Corp BioMed Res Internat Article ID 353074: 1-13.

Jeger V, Brandt S, Porta F, Jakob SM, Takala J, Djafarzadeh S (2014) Hindawi Pub Corp BioMed Res Internat

Abstract: Results on mitochondrial dysfunction in sepsis are controversial. We aimed to assess effects of LPS at wide dose and time ranges on hepatocytes and isolated skeletal muscle mitochondria. Human hepatocellular carcinoma cells (HepG2) were exposed to placebo or LPS (0.1, 1, and 10 𝜇g/mL) for 4, 8, 16, and 24 hours and primary human hepatocytes to 1 𝜇g/mL LPS or placebo (4, 8, and 16 hours). Mitochondria from porcine skeletal muscle samples were exposed to increasing doses of LPS (0.1–100 𝜇g/mg) for 2 and 4 hours. Respiration rates of intact and permeabilized cells and isolated mitochondria were measured by high-resolution respirometry.

In HepG2 cells, LPS reduced mitochondrial membrane potential and cellularATP content but did not modify basal respiration. Stimulated complex II respiration was reduced time-dependently using 1 𝜇g/mL LPS. In primary human hepatocytes, stimulated mitochondrial complex II respiration was reduced time-dependently using 1 𝜇g/mL LPS. In isolated porcine skeletal muscle mitochondria, stimulated respiration decreased at high doses (50 and 100 𝜇g/mL LPS).

LPS reduced cellular ATP content of HepG2 cells, most likely as a result of the induced decrease in membrane potential. LPS decreased cellular and isolated mitochondrial respiration in a time-dependent, dose-dependent and complex-dependent manner.

• O2k-Network Lab: CH Bern Djafarzadeh S

Labels: MiParea: Respiration  Pathology: Sepsis 

Organism: Human  Tissue;cell: Skeletal muscle, Liver  Preparation: Permeabilized cells, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k 

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