Kaambre 2015 Abstract MiP2015

Quantitative characterization of respiratory parameters of human colorectal and breast cancer clinical material.

Link:

Shevchuk I, Koit A, Kaldma A, Chekulayev V, Klepinin A, Ounpuu L, Timohhina N, Tepp K, Kutner R, Heck K, Valvere V, Kaambre T (2015)

Event: MiP2015

Labels:

MiP2015 

Affiliations

1-Lab Bioenergetics, Nat Inst Chemical Physics Biophysics; 2-Oncology Hematology Clinic North Estonia Medical Centre; 3-Tallinn Univ Technology; 4-Tallinn Univ, Inst Mathematics Natural Sciences; Tallinn, Estonia. - [email protected]

Figures

Figure 1. Respiratory chain analysis of HBC, HCC and healthy control breast and colon tissues.

Figure 2. Analysis of the dependence of the respiration rates on the exogenously added ADP in double reciprocal plots: HCC and two populations of mitochondria in control tissue (A) and two different populations of mitochondria in HBC (B).

References and acknowledgements

1. Saks VA, Veksler VI, Kuznetsov AV, Kay L, Sikk P, Tiivel T, Tranqui L, Olivares J, Winkler K, Wiedemann F, Kunz WS (1998) Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem 184:81-100.
2. Kaambre T, Chekulayev V, Shevchuk I, Karu-Varikmaa M, Timohhina N, Tepp K, Bogovskaja J, Kütner R, Valvere V, Saks V (2012) Metabolic control analysis of cellular respiration in situ in intraoperational samples of human breast cancer. J Bioenerg Biomembr 44:539-58.
3. Martinez-Outschoorn UE, Whitaker-Menezes D, Valsecchi M, Martinez-Cantarin MP, Dulau-Florea A, Gong J, Howell A, Flomenberg N, Pestell RG, Wagner J, Arana-Yi C, Sharma M, Sotgia F, Lisanti MP (2013) Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome? Semin Oncol 40:403-18.
4. Witkiewicz AK, Whitaker-Menezes D, Dasgupta A, Philp NJ, Lin Z, Gandara R, Sneddon S, Martinez-Outschoorn UE, Sotgia F, Lisanti MP (2012) Using the "reverse Warburg effect" to identify high-risk breast cancer patients: stromal MCT4 predicts poor clinical outcome in triple-negative breast cancers. Cell Cycle 11:1108-17.