Kudin 1999 Brain Res Brain Res Protoc: Difference between revisions
No edit summary |
No edit summary |
||
Line 11: | Line 11: | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|organism=Rat | |organism=Rat | ||
|tissues= | |tissues=Nervous system | ||
|preparations=Intact Organ | |preparations=Intact Organ | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
}} | }} |
Revision as of 10:06, 27 February 2013
Kudin A, Vielhaber S, Beck H, Elger CE, Kunz WS (1999) Quantitative investigation of mitochondrial function in single rat hippocampal slices: a novel application of high-resolution respirometry and laser-excited fluorescence spectroscopy. Brain Res Brain Res Protoc 4: 329-334. |
Kudin A, Vielhaber S, Beck H, Elger CE, Kunz WS (1999) Brain Res Protoc
Abstract: Highly sensitive techniques are needed for the quantitative determination of mitochondrial oxidative phosphorylation function in single rat hippocampal slices or isolated hippocampal subfields. We determined the oxygen consumption of single hippocampal slices or subfields applying high-resolution respirometry adapted for slice measurements and measured the redox state of mitochondrial NAD(P)H in single hippocampal slices by laser-excited fluorimetry. These methods allow the sensitive detection of two parameters of mitochondrial oxidative phosphorylation which depend on supply of substrates and respiratory chain function. โข Keywords: Hippocampal slice, Hippocampal subfield, Mitochondrial oxidative phosphorylation
Labels:
Organism: Rat
Tissue;cell: Nervous system
Preparation: Intact Organ"Intact Organ" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Coupling state: OXPHOS
HRR: Oxygraph-2k