Malonate: Difference between revisions
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'''Oxygraph-2k Manual Titrations:'''ย [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:'''ย [[MiPNet09.12]] | ||
::* Titration volume: 5 ยตl using a | ::* Titration volume: 5 ยตl using a 25 ยตl syringe | ||
::* Final conc. in 2 ml O2k-chamber: 5 mM | ::* Final conc. in 2 ml O2k-chamber: 5 mM |
Revision as of 09:10, 18 May 2012
Description
Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).
Abbreviation: Mna
Reference: MiPNet09.12
MitoPedia topics:
Inhibitor
Application in HRR
Mna: Malonate (Malonic acid, CH2(COOH)2); Sigma M 1296, 5 g, store at R.T.; FW = 104.06
Preparation of 2 M stock solution (dissolved in H20): Recommended to prepare fresh every day
- 1) weigh 20.8 mg of Malonic acid directly into a Eppendorf tube of appropriate volume (final volume = 100 ยตl)
- 2) dissolve in 20 ยตl H2O
- 3) add 70 ยตl 5 N KOH and check pH
- 4) titrate small amounts (2 ยตl) of 5 N KOH until you reach a pH of 6.0 (approx. 4 titration steps). A pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very little amounts of excess KOH.
- 5) add H2O to a final volume of 100 ยตl
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 ยตl using a 25 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 5 mM