Difference between revisions of "Mason 2008 Methods Enzymol"

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Mason MG, Holladay RS, Nicholls P, Shepherd M, Cooper CE (2008) A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain. Methods Enzymol 437:136-60.

» PMID: 18433627;Open Access

Mason MG, Holladay RS, Nicholls P, Shepherd M, Cooper CE (2008) Methods Enzymol

Abstract: Inhibition of terminal respiratory oxidases by nitric oxide (NO) plays important physiological roles in signaling and host defense. Using a bacterial quinol oxidase and mitochondrial cytochrome c oxidase, this chapter describes simple polarographic methods to quantify the kinetic characteristics of inhibition by NO. This chapter points out the inherent pitfalls of both experimental design and data analysis and compares alternative methods. Additionally, it describes a system designed to acquire polarographic and spectral data simultaneously to permit identification of spectral intermediates under defined conditions.

Labels: MiParea: Respiration

HRR: Oxygraph-2k

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 {{Publication
 |title=Mason MG, Holladay RS, Nicholls P, Shepherd M, Cooper CE (2008) A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain. Methods Enzymol 437:136-60.
-|info=[http://www.ncbi.nlm.nih.gov/pubmed/18433627 PMID: 18433627];[http://www.sciencedirect.com/science/article/pii/S0076687907370080 Open Access]
+|info=[http://www.ncbi.nlm.nih.gov/pubmed/18433627 PMID: 18433627];[http://www.sciencedirect.com/science/article/pii/S0076687907370080  Open Access]
 |authors=Mason MG, Holladay RS, Nicholls P, Shepherd M, Cooper CE
 |year=2008