Mastronicola 2011 IUBMB Life: Difference between revisions
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{{Publication | {{Publication | ||
|title=Mastronicola D, Giuffrรจ A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P (2011) Giardia intestinalis escapes oxidative stress by colonizing the small intestine: A molecular hypothesis. IUBMB Life 63: 21- | |title=Mastronicola D, Giuffrรจ A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P (2011) Giardia intestinalis escapes oxidative stress by colonizing the small intestine: A molecular hypothesis. IUBMB Life 63:21-5. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/21280173 PMID:21280173] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/21280173 PMID: 21280173] | ||
|authors=Mastronicola D, Giuffre A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P | |authors=Mastronicola D, Giuffre A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P | ||
|year=2011 | |year=2011 | ||
|journal=IUBMB Life | |journal=IUBMB Life | ||
|abstract=''Giardia intestinalis'' is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. ''Giardia'' possesses an O<sub>2</sub> -scavenging activity likely essential for survival in the host. We report that Giardia trophozoites express the O<sub>2</sub> -detoxifying flavodiiron protein (FDP), detected by immunoblotting, and are able to reduce O<sub>2</sub> to H<sub>2</sub>O rapidly (โผ3 ฮผM O<sub>2</sub> ร min ร 10<sup>6</sup> cells at 37 ยฐC) and with high affinity (C<sub>50</sub> = 3.4 ยฑ 0.7 ฮผM O<sub>2</sub>). Following a short-term (minutes) exposure to H<sub>2</sub>O<sub>2</sub> โฅ 100 ฮผM, the O<sub>2</sub> consumption by the parasites is irreversibly impaired, and the FDP undergoes a degradation, prevented by the proteasome-inhibitor MG132. Instead, H<sub>2</sub>O<sub>2</sub> does not cause degradation or inactivation of the isolated FDP. On the basis of the elevated susceptibility of ''Giardia'' to oxidative stress, we hypothesize that the parasite preferentially colonizes the small intestine since, compared with colon, it is characterized by a greater capacity for redox buffering and a lower propensity to oxidative stress. | |abstract=''Giardia intestinalis'' is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. ''Giardia'' possesses an O<sub>2</sub> -scavenging activity likely essential for survival in the host. We report that Giardia trophozoites express the O<sub>2</sub> -detoxifying flavodiiron protein (FDP), detected by immunoblotting, and are able to reduce O<sub>2</sub> to H<sub>2</sub>O rapidly (โผ3 ฮผM O<sub>2</sub> ร min ร 10<sup>6</sup> cells at 37 ยฐC) and with high affinity (C<sub>50</sub> = 3.4 ยฑ 0.7 ฮผM O<sub>2</sub>). Following a short-term (minutes) exposure to H<sub>2</sub>O<sub>2</sub> โฅ 100 ฮผM, the O<sub>2</sub> consumption by the parasites is irreversibly impaired, and the FDP undergoes a degradation, prevented by the proteasome-inhibitor MG132. Instead, H<sub>2</sub>O<sub>2</sub> does not cause degradation or inactivation of the isolated FDP. On the basis of the elevated susceptibility of ''Giardia'' to oxidative stress, we hypothesize that the parasite preferentially colonizes the small intestine since, compared with colon, it is characterized by a greater capacity for redox buffering and a lower propensity to oxidative stress. | ||
|keywords=Giardia intestinalis, | |keywords=Giardia intestinalis, Oxygen consumption, Flavoprotein, Detoxifying enzyme, Oxidative stress, Cell proteolysis, Protozoan pathogen | ||
|mipnetlab=IT | |mipnetlab=IT Rome Sarti P | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|injuries=Oxidative stress;RONS | |||
|organism=Protists | |||
|preparations=Intact cells | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 11:20, 21 January 2020
| Mastronicola D, Giuffrรจ A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P (2011) Giardia intestinalis escapes oxidative stress by colonizing the small intestine: A molecular hypothesis. IUBMB Life 63:21-5. |
Mastronicola D, Giuffre A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P (2011) IUBMB Life
Abstract: Giardia intestinalis is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. Giardia possesses an O2 -scavenging activity likely essential for survival in the host. We report that Giardia trophozoites express the O2 -detoxifying flavodiiron protein (FDP), detected by immunoblotting, and are able to reduce O2 to H2O rapidly (โผ3 ฮผM O2 ร min ร 106 cells at 37 ยฐC) and with high affinity (C50 = 3.4 ยฑ 0.7 ฮผM O2). Following a short-term (minutes) exposure to H2O2 โฅ 100 ฮผM, the O2 consumption by the parasites is irreversibly impaired, and the FDP undergoes a degradation, prevented by the proteasome-inhibitor MG132. Instead, H2O2 does not cause degradation or inactivation of the isolated FDP. On the basis of the elevated susceptibility of Giardia to oxidative stress, we hypothesize that the parasite preferentially colonizes the small intestine since, compared with colon, it is characterized by a greater capacity for redox buffering and a lower propensity to oxidative stress. โข Keywords: Giardia intestinalis, Oxygen consumption, Flavoprotein, Detoxifying enzyme, Oxidative stress, Cell proteolysis, Protozoan pathogen
โข O2k-Network Lab: IT Rome Sarti P
Labels:
Stress:Oxidative stress;RONS Organism: Protists
Preparation: Intact cells
HRR: Oxygraph-2k
