Difference between revisions of "MiP03"
From Bioblast
| (2 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=MiP03 | |abbr=MiP03 | ||
|description='''Mitochondrial Preservation Medium, MiP03''', developed for preservation of [[isolated mitochondria]]. | |description='''Mitochondrial Preservation Medium, MiP03''', developed for preservation of [[isolated mitochondria]]. | ||
|info=[[ | |info=[[MiPNet03.02 Chemicals-Media]]; [[Gnaiger 2000 Life in the Cold]] | ||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia concepts|type=Chemicals | ||
| | }} | ||
|type=Chemicals | {{MitoPedia methods|type=Chemicals | ||
}} | |||
{{MitoPedia O2k and high-resolution respirometry|type=Chemicals | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
| Line 13: | Line 15: | ||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
* For preparation of MiP03 see [[MiPNet03.02 Chemicals-Media]]. | |||
* For preparation of MiP03 see [[ | * '''2 mM (+)-α-Tocopherol acid succinate''' (T3126, Sigma Aldrich, store at RT): dissolve 1.0616 mg in 1 ml Ethanol (100%) and store aliquots at 4 °C in dark glass vials (stable for several months) | ||
* '''2 mM (+)-α-Tocopherol acid succinate''' (T3126, Sigma Aldrich): dissolve 1.0616 mg in 1 ml Ethanol (100%) and store aliquots at 4 °C | * '''1 mM Leupeptine''' (L9783, Sigma Aldrich, store at -20 °C): dissolve 0.463 mg in 1 ml H<sub>2</sub>O and store frozen aliquots at -20 °C (at least stable for 6 months) | ||
* '''1 mM Leupeptine''' (L9783, Sigma Aldrich): dissolve 0.463 mg in 1 ml H<sub>2</sub>O and store frozen aliquots at -20 °C | |||
Latest revision as of 16:23, 8 February 2016
- high-resolution terminology - matching measurements at high-resolution
MiP03
Description
Mitochondrial Preservation Medium, MiP03, developed for preservation of isolated mitochondria.
Abbreviation: MiP03
Reference: MiPNet03.02 Chemicals-Media; Gnaiger 2000 Life in the Cold
MitoPedia topics: Media for respirometry
- For preparation of MiP03 see MiPNet03.02 Chemicals-Media.
- 2 mM (+)-α-Tocopherol acid succinate (T3126, Sigma Aldrich, store at RT): dissolve 1.0616 mg in 1 ml Ethanol (100%) and store aliquots at 4 °C in dark glass vials (stable for several months)
- 1 mM Leupeptine (L9783, Sigma Aldrich, store at -20 °C): dissolve 0.463 mg in 1 ml H2O and store frozen aliquots at -20 °C (at least stable for 6 months)
