Difference between revisions of "MiP03"
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* For preparation of MiP03 see [[MiPNet14.13 Medium-MiR06]]. | * For preparation of MiP03 see [[MiPNet14.13 Medium-MiR06]]. | ||
* '''2 mM (+)-α-Tocopherol acid succinate''' (T3126, Sigma Aldrich): dissolve 1.0616 mg in 1 ml Ethanol (100%) and store aliquots at 4 °C | * '''2 mM (+)-α-Tocopherol acid succinate''' (T3126, Sigma Aldrich, store at RT): dissolve 1.0616 mg in 1 ml Ethanol (100%) and store aliquots at 4 °C in dark glass vials (stable for several months) | ||
* '''1 mM Leupeptine''' (L9783, Sigma Aldrich): dissolve 0.463 mg in 1 ml H<sub>2</sub>O and store frozen aliquots at -20 °C | * '''1 mM Leupeptine''' (L9783, Sigma Aldrich, store at -20 °C): dissolve 0.463 mg in 1 ml H<sub>2</sub>O and store frozen aliquots at -20 °C (at least stable for 6 months) | ||
Revision as of 15:05, 20 June 2013
- high-resolution terminology - matching measurements at high-resolution
MiP03
Description
Mitochondrial Preservation Medium, MiP03, developed for preservation of isolated mitochondria.
Abbreviation: MiP03
Reference: MiPNet14.13 Medium-MiR06; Gnaiger 2000 Life in the Cold
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
- For preparation of MiP03 see MiPNet14.13 Medium-MiR06.
- 2 mM (+)-α-Tocopherol acid succinate (T3126, Sigma Aldrich, store at RT): dissolve 1.0616 mg in 1 ml Ethanol (100%) and store aliquots at 4 °C in dark glass vials (stable for several months)
- 1 mM Leupeptine (L9783, Sigma Aldrich, store at -20 °C): dissolve 0.463 mg in 1 ml H2O and store frozen aliquots at -20 °C (at least stable for 6 months)
