Difference between revisions of "MiPNet08.12 IOC22"
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|injuries=Hypoxia | |injuries=Hypoxia | ||
|organism=Rat | |organism=Rat | ||
|tissues= | |tissues=Liver | ||
|preparations=Isolated Mitochondria | |preparations=Isolated Mitochondria | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS |
Revision as of 17:53, 25 February 2013
Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM. NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12. |
Β» MiPNet08.12
Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (
Abstract: A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW
Labels:
Stress:Hypoxia Organism: Rat Tissue;cell: Liver Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Coupling state: OXPHOS
HRR: Oxygraph-2k, NO, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo