MiPNet08.12 IOC22: Difference between revisions
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|authors=Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM | |authors=Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM | ||
|year=* | |year=* | ||
|journal=Mitochondr. Physiol. Network | |||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS | ||
|abstract=A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa). | |||
|info=[http://www.oroboros.at/index.php?id=protocols_no MiPNet08.12] | |info=[http://www.oroboros.at/index.php?id=protocols_no MiPNet08.12] | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|injuries=Hypoxia | |||
|organism=Rat | |organism=Rat | ||
|tissues=Hepatocyte; Liver | |tissues=Hepatocyte; Liver | ||
|preparations=Isolated Mitochondria | |preparations=Isolated Mitochondria | ||
|kinetics=Oxygen, Inhibitor; Uncoupler | |kinetics=Oxygen, Inhibitor; Uncoupler | ||
|topics=Respiration; OXPHOS; ETS Capacity | |topics=Respiration; OXPHOS; ETS Capacity | ||
|instruments=Oxygraph-2k, Method | |||
|articletype=Protocol; Manual, MiPNet-online Publication | |||
}} | }} | ||
[[Category:OroboroPedia]] |
Revision as of 11:14, 20 October 2010
Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM. NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr. Physiol. Network 08.12. |
ยป MiPNet08.12
Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (
) Mitochondr. Physiol. Network
Abstract: A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
Stress:Hypoxia Organism: Rat Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k, Method"Method" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.