Difference between revisions of "MiPNet10.04 CellRespiration"

Latest revision as of 12:51, 4 December 2019

MiPNet10.04 CellRespiration

An experiment with high-resolution respirometry: coupling control in cell respiration.

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Oroboros (2016-08-08) Mitochondr Physiol Network

Abstract: Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.

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Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Blood cells Preparation: Intact cells

Regulation: Coupling efficiency;uncoupling, Uncoupler Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k, TIP2k, O2k-Protocol

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+{{OROBOROS header page name}}
 {{Publication
-|title=Garedew A, Haffner B, Huetter E, Gnaiger E (2005) An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04.
-|info=[[Media:MiPNet10.04 CellRespiration.pdf|'''Open Access-MiPNet10.04.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] An experiment with high-resolution respirometry: coupling control in cell respiration.
-|authors=OROBOROS
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/a/a6/MiPNet10.04_CellRespiration.pdf |Bioblast pdf]]  »[http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions]
-|year=2005
+|authors=Oroboros
+|year=2016-08-08
 |journal=Mitochondr Physiol Network
-|abstract='''O2k-Protocol for Phosphorylation Control in Cell Respiration'''
+|abstract=Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.
-Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS
-Oxygraph-2k (O2k). The experiment demonstrates manual titrations of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).
-:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]
-:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
-|mipnetlab=AT_Innsbruck_OROBOROS
+{{MiPNet pdf page linking to MitoPedia}}
+:» Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]
+|mipnetlab=AT Innsbruck Oroboros
 }}
 {{Labeling
 |area=Respiration, Instruments;methods
@@ Line 21: / Line 22: @@
 |preparations=Intact cells
 |topics=Coupling efficiency;uncoupling, Uncoupler
-|couplingstates=LEAK, ROUTINE, ETS
+|couplingstates=LEAK, ROUTINE, ET
-|substratestates=ROX
+|pathways=ROX
-|instruments=Oxygraph-2k, TIP2k, Protocol
+|instruments=Oxygraph-2k, TIP2k, O2k-Protocol
-|additional=O2k-Demo
 }}
-== Further information ==
-:>> [http://www.oroboros.at/?protocols_cell_hrr MiPNet10.04]
-* Version 6: 2012-04-07
-* Version 1: 2005
-* [[Gnaiger 2012 MitoPathways References|References]]
-[[Category:OroboPedia]]