Difference between revisions of "MiPNet10.04 CellRespiration"
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|title=Garedew A, Haffner B, Huetter E, Gnaiger E. An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04. | |title=Garedew A, Haffner B, Huetter E, Gnaiger E. An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04. | ||
|info=[http://www.oroboros.at/index.php?protocols_cell_hrr MiPNet10.04] | |info=[http://www.oroboros.at/index.php?protocols_cell_hrr MiPNet10.04] | ||
|authors= | |authors=OROBOROS | ||
|year=* | |year=* | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
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}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Instruments and methods | |||
|organism=Mouse | |organism=Mouse | ||
|tissues=Blood cells | |tissues=Blood cells | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|couplingstates= | |couplingstates=LEAK, ROUTINE, ETS | ||
|kinetics= | |kinetics=Uncoupler | ||
|instruments=Oxygraph-2k, TIP2k, Protocol | |instruments=Oxygraph-2k, TIP2k, Protocol | ||
|additional=O2k-Demo | |additional=O2k-Demo | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
Revision as of 11:46, 10 August 2013
| Garedew A, Haffner B, Huetter E, Gnaiger E. An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04. |
OROBOROS (
Abstract: Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Respiration, Instruments and methods"Instruments and methods" is not in the list (Respiration, Instruments;methods, mt-Biogenesis;mt-density, mt-Structure;fission;fusion, mt-Membrane, mtDNA;mt-genetics, nDNA;cell genetics, Genetic knockout;overexpression, Comparative MiP;environmental MiP, Gender, ...) of allowed values for the "MiP area" property.
Organism: Mouse
Tissue;cell: Blood cells
Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, TIP2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
