Difference between revisions of "MiPNet10.04 CellRespiration"
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|abstract='''Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.''' »[http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions] | |abstract='''Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12.''' »[http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions] | ||
Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS O2k. The experiment demonstrates manual [[MiPNet09.12 O2k-Titrations |titrations]] of inhibitors, and automatic titrations of an uncoupler using the electronic [[O2k-Catalogue: TIP2k |Titration-Injection microPump TIP2k]]. Application of the DatLab 7 software is shown for instrumental control (O2k and TIP2k), and [[MiPNet19.18E O2 | Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS O2k. The experiment demonstrates manual [[MiPNet09.12 O2k-Titrations |titrations]] of inhibitors, and automatic titrations of an uncoupler using the electronic [[O2k-Catalogue: TIP2k |Titration-Injection microPump TIP2k]]. Application of the DatLab 7 software is shown for instrumental control (O2k and TIP2k), and [[MiPNet19.18E O2-flux analysis |real-time data analysis]]. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the O2k Manual. The experiments were carried out by participants of an O2k-Workshop on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria). | ||
:>> Product: [[Oxygraph-2k]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]] | :>> Product: [[Oxygraph-2k]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]] | ||
|mipnetlab=AT Innsbruck OROBOROS | |mipnetlab=AT Innsbruck OROBOROS |
Revision as of 12:49, 14 August 2016
An experiment with high-resolution respirometry: coupling control in cell respiration. |
OROBOROS (2016-08-08) Mitochondr Physiol Network
Abstract: Doerrier C, Gnaiger E (2005-2016) An experiment with high-resolution respirometry: Coupling control in cell respiration. Mitochondr Physiol Network 10.04(09):1-12. »Versions
Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS O2k. The experiment demonstrates manual titrations of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k. Application of the DatLab 7 software is shown for instrumental control (O2k and TIP2k), and real-time data analysis. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the O2k Manual. The experiments were carried out by participants of an O2k-Workshop on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).
- >> Product: Oxygraph-2k, O2k-Catalogue
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Blood cells
Preparation: Intact cells
Regulation: Coupling efficiency;uncoupling, Uncoupler Coupling state: LEAK, ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, TIP2k, O2k-Protocol
O2k-Demo, O2k-Core
DatLab-Excel templates and DatLab Demo Files
DatLab 7
- Calibration-File: MiPNet10.04_CellRespiration_Calib.DLD
- Demo-File: MiPNet10.04_CellRespiration.DLD
- Chemical Background Excel demo and template: MiPNet10.04_CellRespiration O2k-ChemBackground.xlsx
- DatLab-Excel template: SUIT_MiPNet10.04_CellRespiration.xlsx