Difference between revisions of "MiPNet10.05 O2-Concentration-Flux"
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{{Publication | {{Publication | ||
|title=Gnaiger E (2005-2010) From oxygen concentration to oxygen flux. Mitochondr Physiol Network 10.05. - ''New edition:'' [[Gnaiger_2012_MitoPathways|Gnaiger 2012 Chapter 1]]. | |title=Gnaiger E (2005-2010) From oxygen concentration to oxygen flux. Mitochondr Physiol Network 10.05. - ''New edition:'' [[Gnaiger_2012_MitoPathways|Gnaiger 2012 Chapter 1]]. | ||
|info=[[ | |info= [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/6c/MiPNet10.05_O2-Concentration-Flux.pdf|Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet10.05_O2-Concentration-Flux.pdf Versions] | ||
|year=2005 | |year=2005 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''O2k-Protocol for Oxygen flux | |abstract='''O2k-Protocol for Oxygen flux''' | ||
In a closed oxygraph chamber, the oxygen concentration declines over time as a result of respiratory processes. The time derivative, therefore, is a negative number. Why is then the ‘rate of oxygen consumption’ not expressed as a negative value? Why is the term ‘oxygen flux’ used in this context of chemical reactions? The rationale is based on fundamental concepts of physical chemistry and non-equilibrium thermodynamics. | In a closed oxygraph chamber, the oxygen concentration declines over time as a result of respiratory processes. The time derivative, therefore, is a negative number. Why is then the ‘rate of oxygen consumption’ not expressed as a negative value? Why is the term ‘oxygen flux’ used in this context of chemical reactions? The rationale is based on fundamental concepts of physical chemistry and non-equilibrium thermodynamics. | ||
[[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] | |||
[[Image:MiPNet10.05.jpg|centre|500px|thumb]] | [[Image:MiPNet10.05.jpg|centre|500px|thumb]] | ||
Respiratory oxygen flux: On-line display of oxygen concentration (blue) and oxygen flux (respiration, red). Endogenous respiration of endothelial cells leads to oxygen depletion, followed by reoxygenations (dotted arrows). Cell membrane permeabilization by digitonin causes a decline of respiration to the resting level (without adenylates in the medium, -ANP). ADP titration activates respiration about 2-fold above the endogenous level of oxygen consumption. | Respiratory oxygen flux: On-line display of oxygen concentration (blue) and oxygen flux (respiration, red). Endogenous respiration of endothelial cells leads to oxygen depletion, followed by reoxygenations (dotted arrows). Cell membrane permeabilization by digitonin causes a decline of respiration to the resting level (without adenylates in the medium, -ANP). ADP titration activates respiration about 2-fold above the endogenous level of oxygen consumption. | ||
Eye-fitted slopes of oxygen chart recorder traces belong to the past. With [ | Eye-fitted slopes of oxygen chart recorder traces belong to the past. With [[DatLab|DatLab]], trends are resolved. Accuracy is improved by standard numerical corrections. Graphs and protocols are stored and printed ready for publication. | ||
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:>> O2k-Protocols:[ | :>> O2k-Protocols:[[O2k-Protocols| Overall contents]] | ||
:>> Product: [ | :>> Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]] | ||
|keywords=Archive | |keywords=Archive | ||
|mipnetlab= | |mipnetlab=AT_Innsbruck_Oroboros | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Protocol | |instruments=O2k-Protocol | ||
|additional=Archive | |additional=Archive | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
}} | }} | ||
== Further Information== | == Further Information== | ||
:>> [ | :>> [[Gnaiger 2012 MitoPathways References]] | ||
Latest revision as of 10:54, 11 January 2018
| Gnaiger E (2005-2010) From oxygen concentration to oxygen flux. Mitochondr Physiol Network 10.05. - New edition: Gnaiger 2012 Chapter 1. |
» 
»Versions
Abstract: O2k-Protocol for Oxygen flux
In a closed oxygraph chamber, the oxygen concentration declines over time as a result of respiratory processes. The time derivative, therefore, is a negative number. Why is then the ‘rate of oxygen consumption’ not expressed as a negative value? Why is the term ‘oxygen flux’ used in this context of chemical reactions? The rationale is based on fundamental concepts of physical chemistry and non-equilibrium thermodynamics.
Respiratory oxygen flux: On-line display of oxygen concentration (blue) and oxygen flux (respiration, red). Endogenous respiration of endothelial cells leads to oxygen depletion, followed by reoxygenations (dotted arrows). Cell membrane permeabilization by digitonin causes a decline of respiration to the resting level (without adenylates in the medium, -ANP). ADP titration activates respiration about 2-fold above the endogenous level of oxygen consumption.
Eye-fitted slopes of oxygen chart recorder traces belong to the past. With DatLab, trends are resolved. Accuracy is improved by standard numerical corrections. Graphs and protocols are stored and printed ready for publication.
Reference
- >> O2k-Protocols: Overall contents
- >> Product: Oroboros O2k, O2k-Catalogue
• Keywords: Archive
• O2k-Network Lab: AT_Innsbruck_Oroboros
Labels:
HRR: O2k-Protocol
Archive
