Difference between revisions of "MiPNet10.05 O2-Concentration-Flux"
| Line 4: | Line 4: | ||
|year=2005 | |year=2005 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract= | |abstract='''O2k-Protocol for Oxygen flux | ||
'''O2k-Protocol for Oxygen flux | |||
In a closed oxygraph chamber, the oxygen concentration declines over time as a result of respiratory processes. The time derivative, therefore, is a negative number. Why is then the ‘rate of oxygen consumption’ not expressed as a negative value? Why is the term ‘oxygen flux’ used in this context of chemical reactions? The rationale is based on fundamental concepts of physical chemistry and non-equilibrium thermodynamics. | In a closed oxygraph chamber, the oxygen concentration declines over time as a result of respiratory processes. The time derivative, therefore, is a negative number. Why is then the ‘rate of oxygen consumption’ not expressed as a negative value? Why is the term ‘oxygen flux’ used in this context of chemical reactions? The rationale is based on fundamental concepts of physical chemistry and non-equilibrium thermodynamics. | ||
FIGURE HERE | |||
Respiratory oxygen flux: On-line display of oxygen concentration (blue) and oxygen flux (respiration, red). Endogenous respiration of endothelial cells leads to oxygen depletion, followed by reoxygenations (dotted arrows). Cell membrane permeabilization by digitonin causes a decline of respiration to the resting level (without adenylates in the medium, -ANP). ADP titration activates respiration about 2-fold above the endogenous level of oxygen consumption. | |||
Eye-fitted slopes of oxygen chart recorder traces belong to the past. With [http://www.oroboros.at/index.php?id=datlab DatLab], trends are resolved. Accuracy is improved by standard numerical corrections. Graphs and protocols are stored and printed ready for publication. | |||
'''Reference''' | |||
[[Gnaiger_1993_Pure_Appl_Chem| Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002.]] | |||
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | :>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
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}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Protocol | |||
|additional=O2k-SOP | |additional=O2k-SOP | ||
| | |articletype=Protocol; Manual, MiPNet-online Publication | ||
}} | }} | ||
== Further Information== | == Further Information== | ||
:>> [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways] | :>> [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways] | ||
:>> [http://www.oroboros.at/index.php?id=o2-conc-flux www Flux] | :>> [http://www.oroboros.at/index.php?id=o2-conc-flux www Flux] | ||
Revision as of 12:06, 29 November 2013
| Gnaiger E (2005-2010) From oxygen concentration to oxygen flux. Mitochondr Physiol Network 10.05. - New edition: Gnaiger 2012 Chapter 1. |
» Open Access-MiPNet10.05.pdf; Versions
Abstract: O2k-Protocol for Oxygen flux In a closed oxygraph chamber, the oxygen concentration declines over time as a result of respiratory processes. The time derivative, therefore, is a negative number. Why is then the ‘rate of oxygen consumption’ not expressed as a negative value? Why is the term ‘oxygen flux’ used in this context of chemical reactions? The rationale is based on fundamental concepts of physical chemistry and non-equilibrium thermodynamics.
FIGURE HERE
Respiratory oxygen flux: On-line display of oxygen concentration (blue) and oxygen flux (respiration, red). Endogenous respiration of endothelial cells leads to oxygen depletion, followed by reoxygenations (dotted arrows). Cell membrane permeabilization by digitonin causes a decline of respiration to the resting level (without adenylates in the medium, -ANP). ADP titration activates respiration about 2-fold above the endogenous level of oxygen consumption.
Eye-fitted slopes of oxygen chart recorder traces belong to the past. With DatLab, trends are resolved. Accuracy is improved by standard numerical corrections. Graphs and protocols are stored and printed ready for publication.
Reference
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• Keywords: Archive
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-SOP
