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Difference between revisions of "MiPNet11.05 imt-pti-pce"

From Bioblast
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:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
|mipnetlab=AT_Innsbruck_OROBOROS}}
|mipnetlab=AT_Innsbruck_OROBOROS
}}
{{Labeling
{{Labeling
|area=mt-Biogenesis;mt-density
|area=mt-Biogenesis;mt-density
|preparations=Permeabilized cells, Permeabilized tissue, Isolated Mitochondria
|preparations=Permeabilized cells, Permeabilized tissue, Isolated mitochondria
|instruments=Protocol
|instruments=Protocol
|additional=Mt-Preparation}}
|additional=Mt-Preparation
}}
==Further information ==
==Further information ==



Revision as of 11:22, 25 February 2015

Publications in the MiPMap
O2k-Protocols
Isolated mitochondria or permeabilized tissues and cells.

Β» Open Access.MiPNet11.05.pdf, Versions

OROBOROS (2014-04) Mitochondr Physiol Network

Abstract: Gnaiger E (2014) Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05(06): 1-5.

Whereas isolated mitochondria remain one of the gold-standards in studies of bioenergetics and mitochondrial physiology, permeabilized tissues and cells have become an alternative with several advantages. But some disadvantages have to be considered, too, for optimum experimental design and critical evaluation of results.

>> O2k-Protocols:Overall contents
>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue


β€’ O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: mt-Biogenesis;mt-density 



Preparation: Permeabilized cells, Permeabilized tissue, Isolated mitochondria 



HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Mt-Preparation 

Further information

We recommend the new METTLER TOLEDO microbalances XSE105DU for determination of tissue wet weight (1 to 3 mg wet weight per chamber). We offer this microbalance as part of our systems-approach to high-resolution respirometry.