MiPNet12.23 FiberRespiration: Difference between revisions
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{{Publication | {{Publication | ||
|title=Lemieux H, Votion DM, Gnaiger E. Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. | |title=Lemieux H, Votion DM, Gnaiger E. Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. | ||
|info=[http://www.oroboros.at/ | |info=[http://www.oroboros.at/?protocols_muscle_horse MiPNet12.23] | ||
|authors=Lemieux H, Votion DM, Gnaiger E, | |authors=Lemieux H, Votion DM, Gnaiger E, | ||
|year=* | |year=* | ||
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software is shown for on-line data analysis [[MiPNet12.09]] The following guideline describes the experimental protocol and includes a short discussion of | software is shown for on-line data analysis [[MiPNet12.09]] The following guideline describes the experimental protocol and includes a short discussion of | ||
results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 (IOC44; Schroecken, Austria). | results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 (IOC44; Schroecken, Austria). | ||
|mipnetlab=AT_Innsbruck_OROBOROS, BE_Liege_Votion DM | |mipnetlab=AT_Innsbruck_OROBOROS, BE_Liege_Votion DM | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
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}} | }} | ||
{{Labeling | {{Labeling | ||
| | |area=Respiration, Instruments and methods | ||
|organism=Horse | |organism=Horse | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
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|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII, CI+II | |substratestates=CI, CII, CI+II | ||
|instruments=Oxygraph-2k, Protocol | |||
|additional=O2k-Demo | |additional=O2k-Demo | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
Revision as of 12:45, 10 August 2013
| Lemieux H, Votion DM, Gnaiger E. Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. |
Lemieux H, Votion DM, Gnaiger E (
Abstract: Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- 2k (O2k). The experiment demonstrates manual titrations applied to study respiratory capacity in permeabilized fibres. Application of the DatLab 4 software is shown for on-line data analysis MiPNet12.09 The following guideline describes the experimental protocol and includes a short discussion of results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 (IOC44; Schroecken, Austria).
• O2k-Network Lab: AT_Innsbruck_OROBOROS, BE_Liege_Votion DM
Labels: MiParea: Respiration, Instruments and methods"Instruments and methods" is not in the list (Respiration, Instruments;methods, mt-Biogenesis;mt-density, mt-Structure;fission;fusion, mt-Membrane, mtDNA;mt-genetics, nDNA;cell genetics, Genetic knockout;overexpression, Comparative MiP;environmental MiP, Gender, ...) of allowed values for the "MiP area" property.
Organism: Horse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
Info
MiPNet04.07 is integrated in MiPNet12.23.
