Difference between revisions of "MiPNet18.13 IOC84 Alaska"

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MiPNet18.13 IOC84 Alaska

Big Lake, Alaska US, 2014 Jan 04-07. Oroboros O2k-Workshop on HRR and Racing dogs at Iditarod, IOC84. »US OK Stillwater Davis MS

» Miller 2017 PLOS ONE

OROBOROS (2014-01-04) Mitochondr Physiol Network

Abstract:

84th Oroboros O2k-Workshop on high-resolution respirometry in cooperation with O2k-Network Lab US OK Stillwater Davis MS.

Project: Racing dogs at Iditarod, Alaska 2014 with integrated O2k-Workshop

• O2k-Network Lab: US OK Stillwater Davis MS, AT Innsbruck OROBOROS

IOC84: Racing dogs at Iditarod, Alaska 2014

A special expertise of the Oroboros mitoscience-team is the logistic planning and support of expeditions with a focus on mitochondrial physiology. IOC84 was integrated in the Alasca expedition as a training course.

>> IOC84

Collaboration of Michael Davis and Katherine Williamson (Comparative Exercise Physiology Laboratory, Oklahoma State University) with Martin Buser (Happy Trails Kennel, Alaska), Robert Boushel (Swedish School of Sport and Health Sciences, Stockholm, SE), Benjamin F. Miller and Karyn Hamilton (Colorado State University, US), and Erich Gnaiger and Verena Laner (Medical University of Innsbruck and Oroboros Instruments) - a Power-O2k project with six Oroboros O2k.

Results published

Data generated in this collaborative study are published: Miller 2017 PLOS ONE.

Lecturers and tutors

Davis Michael S, Professor, Physiological Sciences Oxley Equine Professor

Director, Comparative Exercise Physiology Lab

Gnaiger Erich, CEO, Oroboros Instruments

Laner Verena; Project manager and application specialist, Oroboros INSTRUMENTS

Boushel RC; Professor and Chair in Physiology, The Swedish School of Sport and Health Sciences

Alaska Expedition pre-study ("Iditarod"). Collaboration with Michael Davis (Oklahoma State University, US), Robert Boushel (Swedish School of Sport and Health Sciences, Stockholm, SE) and Benjamin F. Miller (Colorado State University, US) - with integrated IOC84 (Jan 04-07).

Dr. Erich Gnaiger (Medical University of Innsbruck and Oroboros Instruments) and his collaborator Verena Laner (Oroboros Instruments) joined the project of Michael Davis (Oklahoma State University) in Big Lake, Alaska. Cumulative training effects on the mitochondrial function of alaskan sled dogs were studied in January and March 2014 at Happy Trails Kennel in Big Lake, Alaska. Further participants were Robert Boushel (Institute for Sport and Health Sciences, Stockholm, SE) as well as Benjamin F. Miller and Karyn Hamilton (Colorado State University, Fort Collins, US).

Racing dogs at Iditarod, Alaska 2014

Iditarod - The last great race(r) 2014: the greatest challenge up to the finish: » iditarod.com

At Martin Buser's 'Happy Trails Kennel' (Big Lake, near Anchorage, Alaska) Prof. Michael Davis (Comparative Exercise Physiology Laboratory, Oklahoma State University, USA) conducts a long-term collaboration with Martin's team to study and train the Alaskan dogs in preparation of long-distance races, the most challenging of which is the 1,100 mile Iditarod race. Martin Buser is 4 times champion of the Iditarod, with a scientifically oriented interest in the study of the genetics and physiology of the dogs that goes far beyond the race.

The line of mixed-breed Alaskan huskies of Martin incorporates sprint-dog lines and is selected and trained as much for long-distance endurance as for speed - and happiness. "Happy dogs perform better and make racing more fun" (Martin Buser).

» buserdog.com

In January and March 2014, Mike Davis and Katherine Williamson teamed up with Erich Gnaiger and Verena Laner (Medical University of Innsbruck and Oroboros Instruments), Robert Boushel (Swedish School of Sport and Health Sciences, Stockholm, SE), and Benjamin F. Miller and Karyn Hamilton (Colorado State University, US) to focus on mitochondrial respiratory function in the skeletal muscle of the dogs. With a Power-O2k approach using six Oroboros O2k, these mitochondrial studies are conducted at the beginning of the intensive training period and after the Iditarod race, considered as the final stage of the most intensive endurance training.

» Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis.

The Iditarod team of fast endurance dogs
Martin Buser (Jan 2014)
'Heavy reading' on a light sled (musher Martin Buser)

The tutors

Verena Laner and Robert Boushel titrating simultaneously into multiple chambers of the Power-O2k array, seeing the muscle fibres through the windows of the O2k-Chambers, while we see the dogs in the kennel through the lab window.
Robert Boushel at muscle fibre preparation with the new Oroboros cooling plate. The snow for cooling BIOPS and MiR06Cr is readily available outside.

The science team

The IOC84 team at Happy Trails Kennel, left to right, back row: Verena Laner, Karyn Hamilton, Robert Boushel, Katherine Williamson, Michael Davis, Shannon Massie; front row: Erich Gnaiger, Benjamin F. Miller.
Left to right, back: Karyn Hamilton, Benjamin F. Miller, Robert Boushel, Katherine Chapoton, Michael Davis, Katherine Williamson, Verena Laner; front: Shannon Massie, Martin Buser, Erich Gnaiger.
Left to right: Shannon Massie, Verena Laner, Karyn Hamilton, Benjamin F. Miller, Katherine Williamson, Michael Davis, Katherine Chapoton, Martin Buser, Erich Gnaiger, Robert Boushel.
The Mito-Science-Team (left to right: Rob Boushel, Erich Gnaiger, Karyn Hamilton, Verena Laner and Benjamin F. Miller) working in the lab. (C) by Shannon Massie.
Getting ready for an exciting sled-ride through the Alaskan nature - Rob Boushel and Rohn Buser.

Impressions of the study

The fast endurance heroes

on
the
treadmill

The mitochondrial lab at Happy Trails Kennel

Dog skeletal muscle mitochondria: the first detailed study with SUIT protocols.

A Power-O2k approach with six Oxygraph-2k in Pelicases and the microbalance in the transport case
Katherine Williamson
Erich Gnaiger
Verena Laner and dog 'Lucky'
Verena Laner, Robert Boushel

Getting connected

What do the dogs tell us?

A visitor at Happy Trails Kennel

Moose - not yet studied in terms of mitochondrial physiology

The mito team

Setting up the mito lab with Martin Buser
Katherine Chapoton, Katherine Williamson and Mike Davis discuss the races.
Mike Davis and Erich Gnaiger discuss the SUIT protocols.

Alaska Expedition main study (March 2014)

Trained cohort

SUIT protocols SUIT_FNS(PGM)03, SUIT_NS(PGM)04

Titrations for SUIT protocols

Left to right: Shannon Massie, Benjamin F. Miller, Erich Gnaiger, Karyn Hamilton, Robert Boushel
Left to right:Benjamin F. Miller, Erich Gnaiger, Karyn Hamilton, Shannon Massie, Robert Boushel

Permeabilized fibre preparation

Karyn Hamilton: only 1.4 to 1.8 mg wet weight were prepared for each O2k-Chamber.
Testing the new Oroboros cooling plate.

Fotos by Gnaiger Erich and Laner Verena

Further links of interest

Youtube
k300: Congratulations to Rohn Buser for winning this race. "Rohn won it in 38 h and change, but not without a MAJOR challenge from Jeff King who made a run at Rohn in the last 5-10 miles, but couldn't overtake him. 300 miles (500 km) in 38 h. When you subtract the 10 h of mandatory rest, that means Rohn's dogs AVERAGED nearly 18 kph for over a day. These are the dogs we will investigate in March. That isn't even the race record. Ironically, the race record of 37 h 4 min is held by Martin Buser" (Michael S. Davis, 2014-01-20).