Difference between revisions of "MiPNet20.14 AmplexRed H2O2-production"

Revision as of 16:44, 15 November 2019

O2k-FluoRespirometry: HRR and simultaneous determination of H₂O₂ production with Amplex UltraRed.

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Oroboros (2019-06-24) Mitochondr Physiol Network

Abstract: Krumschnabel G, Fontana-Ayoub M, Fasching M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and simultaneous determination of H₂O₂ production with Amplex UltraRed. Mitochondr Physiol Network 20.14(04):1-6.

• O2k-Network Lab: AT_Innsbruck_Oroboros

Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Heart Preparation: Isolated mitochondria

Coupling state: LEAK, OXPHOS, ET Pathway: S HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol

O2k-Demo, O2k-MultiSensor, MitoFitPublication, SUIT-013, SUIT-013 AmR ce D023, SUIT-009, SUIT-009 AmR mt D021, SUIT-009 AmR pce D019, SUIT-018, SUIT-018 AmR mt D031, SUIT-006 AmR mt D048, SUIT-003 AmR ce D017, SUIT-003 AmR ce D058

Template:Keywords H2O2

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 |year=2019-06-24
 |journal=Mitochondr Physiol Network
-|abstract='''Krumschnabel G, Fontana-Ayoub M, Fasching M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and simultaneous determination of H<sub>2</sub>O<sub>2</sub> production with Amplex UltraRed. Mitochondr Physiol Network 20.14(04):1-6.'''
+|abstract=Krumschnabel G, Fontana-Ayoub M, Fasching M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and simultaneous determination of H<sub>2</sub>O<sub>2</sub> production with Amplex UltraRed. Mitochondr Physiol Network 20.14(04):1-6.
-[[High-resolution respirometry]] is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluorometry_-_Fluorophores|H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels]].
-Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
-# Quality control of preparations by respirometric performance.
-# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.
-# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
-# Additional information is acquired for a limited amount of sample.
-# Oxygen dependence of parameters can be studied.
-In the publication by [[Krumschnabel_2015_Methods_Mol_Biol|Krumschnabel et al. 2015]] we introduce the O2k-Fluo LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H<sub>2</sub>O<sub>2</sub> production of mouse brain mitochondria. The paper by [[Makrecka-Kuka_2015_Biomolecules|Makrecka-Kuka et al. 2015]] focuses on the application of the Amplex red assay for determining H<sub>2</sub>O<sub>2</sub> production in three different models of mitochondrial preparations.
-'''O2k-Publication''':
-[[Image:O2k-Publications.jpg|50px|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]]
-Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. [[Krumschnabel 2015 Methods Mol Biol |»Bioblast link«]]
-[[Image:O2k-Publications.jpg|50px|left|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]]
-Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. [[Makrecka-Kuka 2015 Biomolecules| »Bioblast link«]]
 |mipnetlab=AT_Innsbruck_Oroboros
 }}