Difference between revisions of "MiPNet22.04 IOC120 Barcelona ES"

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MiPNet22.04 IOC120 Barcelona ES

Barcelona ES, 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry IOC120. »ES Barcelona Zorzano A

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Abstract:

O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry - satellite to MITOEAGLE Barcelona 2017. Barcelona, Spain; 2017 Mar 20.

Monday 20th	Topic	Weblink
08:45	Registration
09:00-09:15	Welcome by Prof. Antonio Zorzano.
09:15-09:30	Introduction of participants and their research interests.	IOC120
09:30-10:00	Get started with the O2k: Overview with video clips.	O2k-Manual
10:00-11:30	Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control by coupling and mitochondrial pathways, SUIT reference assay.	The Blue Book - SUIT reference protocol
11:30-12:00	Coffee/tea break
12:00-13:00	Principles of high-resolution respirometry: From switching on the O2k to the experimental result – oxygen sensor calibration and quality control.	POS-calibration-SOP
13:00-14:00	O2k-Fluorometry and OXPHOS analysis: Amplex red assay of H2O2 production and design of experimental protocol.	O2k-Fluorometry
14:00-15:00	Lunch
15:00-17:30	Demo-Experiment: HRR and O2k-Fluorometry with permeabilized cells – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2.	RP1 - RP2
17:30-18:30	Protocol driver for Marks and data analysis.
18:30-20:00	The Bioblast wiki, O2k-Network and feedback discussion.

1 Torre D (Room 1 Tower D)

R+D+I Towers building

Parc Científic de Barcelona

Barcelona Science Park

C/ Baldiri Reixac 4-8

08028 Barcelona

To get to the workhsop location following routes are possible:

Start in Barcelona centrum: easiest way is to take the Metro Line 3 (L3) to Palau Reial and walk approximately 5 min to the final destination

Start airport: take the Metro Line 9 (L9) to Zona Universitaria, which will take around 30 min

Taxi will take about 20 min and the costs are approximately 20-25 €
Another option is the train (Renfe to Sants Station) and then there the Metro L3 to Palau Reial

O2k-Fluorometer demo experiment: high-resolution respirometry and H₂O₂ production (Amplex red)
Followed by the Barcelona MITOEAGLE Conference, MG and WC Meeting.

OROBOROS INSTRUMENTS

high-resolution respirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Mitochondria and cell research

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.

» IOC recommended reading

O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.

Labels:

HRR: Oxygraph-2k

ORO, IOC, 2017, Next, O2k-Network

@@ Line 12: / Line 12: @@
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 __TOC__
 == Programme ==
@@ Line 72: / Line 73: @@
 == Venue ==
-:::: Institute for Research in Biomedicine (IRB Barcelona)
+::::1 Torre D (Room 1 Tower D)
-:::: Facultat de Biologia
+::::R+D+I Towers building
-:::: Universitat de Barcelona
+::::Parc Científic de Barcelona
-:::: c/ Baldiri Reixac, 10-12, 08028
+::::Barcelona Science Park
-:::: Barcelona, Spain
+::::C/ Baldiri Reixac 4-8
+::::08028 Barcelona
+::::To get to the workhsop location following routes are possible:
+::::*Start '''in Barcelona centrum''': easiest way is to take the Metro Line 3 (L3) to Palau Reial and walk approximately 5 min to the final destination
+:::::*Another option is to take a bus(see picture below with bus plan)
+::::*Start '''airport''': take the Metro Line 9 (L9) to Zona Universitaria, which will take around 30 min
+:::::*Taxi will take about 20 min and the costs are approximately 20-25 €
+:::::*Another option is the train (Renfe to Sants Station) and then there the Metro L3 to Palau Reial
+<gallery mode=default perrow=4 widths="300px" heights="300px">
+File:Pcbmap.jpg
+File:Mapa situacio pcb.jpg
+File:Mapab.jpg
+</gallery>
 == General information ==