MiPNet22.04 IOC120 Barcelona ES

MiPNet22.04 IOC120 Barcelona ES

Barcelona ES, 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry IOC120. »ES Barcelona Zorzano A

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Abstract:

O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry - satellite to MITOEAGLE Barcelona 2017. Barcelona, Spain; 2017 Mar 20.

General information

O2k-Fluorometer demo experiment: high-resolution respirometry and H₂O₂ production (Amplex red)
Followed by the Barcelona MITOEAGLE Conference, MG and WC Meeting.

Monday 20th	Topic	Weblink
08:45	Registration
09:00-09:15	Welcome by Prof. Antonio Zorzano.
09:15-09:30	Introduction of participants and their research interests.	IOC120
09:30-10:00	Get started with the O2k: Overview with video clips.	O2k-Manual
10:00-11:30	Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control by coupling and mitochondrial pathways, SUIT reference assay.	The Blue Book - SUIT reference protocol
11:30-12:00	Coffee/tea break
12:00-13:00	Principles of high-resolution respirometry: From switching on the O2k to the experimental result – oxygen sensor calibration and quality control.	POS-calibration-SOP
13:00-14:00	O2k-Fluorometry and OXPHOS analysis: Amplex red assay of H2O2 production and design of experimental protocol.	O2k-Fluorometry
14:00-15:00	Lunch
15:00-17:30	Demo-Experiment: HRR and O2k-Fluorometry with liver mitochondria – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2.	RP1 - RP2
17:30-18:30	Protocol driver for Marks and data analysis.
18:30-20:00	The Bioblast wiki, O2k-Network and feedback discussion.

Barcelona Science Park

Room 1 Tower D

R+D+I Towers building

C/ Baldiri Reixac 4-8

08028 Barcelona

To get to the workhsop location following routes are possible:

Start in Barcelona centrum: easiest way is to take the Metro Line 3 (L3) to Palau Reial and walk approximately 5 min to the final destination

Start airport: take the Metro Line 9 (L9) to Zona Universitaria, which will take around 30 min

Taxi will take about 20 min and the costs are approximately 20-25 €
Another option is the train (Renfe to Sants Station) and then there the Metro L3 to Palau Reial

	Participant	Institution
	Antje Garten	UK_Birmingham_Manjeri_G: Institute of Metabolism and Systems Research, University of Birmingham (UK)
	Laura Herrero	Dpt. Biochemistry. School of Pharmacy. Univ. Barcelona, University of Barcelona (ES)
	Pau Gama	ES Barcelona Garcia-Roves PM : Physiological Science Department, University of Barcelona (ES)
	Alexander Lyakhovich	Research Institute VHIR, Barcelona (ES)
	David Sebastián	ES_Barcelona_Zorzano A: IRB Barcelona (ES)
	Sebastián Zagmutt Caroca	University of Barcelona (ES)
	Matteo Giovarelli	IT_Milan_Clementi_E: Department of Biomedical and Clinical Sciences University of Milan, University of Milan (IT)
	Rubén Martín Cabezuelo	Universitat Politècnica de València (UPV)
	Lara Nogueras	ES Lleida Boada J: IRB Lleida (ES)
	Hugo Gonzalo	ES Lleida Boada J: IRB Lleida (ES)
	Javier Iglesias-Gonzalez	UK_Manchester_Galli GL: Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester (UK)

OROBOROS INSTRUMENTS

high-resolution respirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Mitochondria and cell research

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.

» IOC recommended reading

O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.

Labels:

HRR: Oxygraph-2k

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