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| |year=2019-08-05 | | |year=2019-08-05 |
| |journal=Mitochondr Physiol Network | | |journal=Mitochondr Physiol Network |
| |abstract='''Komlodi T, Iglesias-Gonzalez J, Cardoso LHD, Sobotka O, Gnaiger E (2019) Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4. Mitochondr Physiol Network 24.10(01):1-5.''' | | |abstract=Komlodi T, Iglesias-Gonzalez J, Cardoso LHD, Sobotka O, Gnaiger E (2019) Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4. Mitochondr Physiol Network 24.10(01):1-5. |
| | | {{MiPNet pdf page linking to MitoPedia}} |
| [[High-resolution respirometry]] is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluorometry_-_Fluorophores|H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels]].
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| Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
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| # Quality control of preparations by respirometric performance.
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| # Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.
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| # Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
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| # Additional information is acquired for a limited amount of sample.
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| # Oxygen dependence of parameters can be studied.
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| In the publication by [[Krumschnabel_2015_Methods_Mol_Biol|Krumschnabel et al. 2015]] we introduce the O2k-Fluo LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H<sub>2</sub>O<sub>2</sub> production of mouse brain mitochondria. The paper by [[Makrecka-Kuka_2015_Biomolecules|Makrecka-Kuka et al. 2015]] focuses on the application of the Amplex red assay for determining H<sub>2</sub>O<sub>2</sub> production in three different models of mitochondrial preparations.
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| '''O2k-Publication''':
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| [[Image:O2k-Publications.jpg|50px|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]]
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| Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. [[Krumschnabel 2015 Methods Mol Biol |»Bioblast link«]]
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| [[Image:O2k-Publications.jpg|50px|left|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]]
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| Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. [[Makrecka-Kuka 2015 Biomolecules| »Bioblast link«]]
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| |mipnetlab=AT_Innsbruck_Oroboros | | |mipnetlab=AT_Innsbruck_Oroboros |
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| |additional=O2k-MultiSensor, MitoFitPublication, SUIT-013, SUIT-013 AmR ce D023, SUIT-009, SUIT-009 AmR mt D021, SUIT-009 AmR pce D019, SUIT-018, SUIT-018 AmR mt D031, SUIT-006 AmR mt D048, SUIT-003 AmR ce D017, SUIT-003 AmR ce D058, O2k-Analysis, DatLab, | | |additional=O2k-MultiSensor, MitoFitPublication, SUIT-013, SUIT-013 AmR ce D023, SUIT-009, SUIT-009 AmR mt D021, SUIT-009 AmR pce D019, SUIT-018, SUIT-018 AmR mt D031, SUIT-006 AmR mt D048, SUIT-003 AmR ce D017, SUIT-003 AmR ce D058, O2k-Analysis, DatLab, |
| }} | | }} |
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| {{Keywords H2O2}}
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MiPNet24.10 H2O2 flux analysis
| Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4
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» 
»Versions
Oroboros (2019-08-05) Mitochondr Physiol Network
Abstract: Komlodi T, Iglesias-Gonzalez J, Cardoso LHD, Sobotka O, Gnaiger E (2019) Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4. Mitochondr Physiol Network 24.10(01):1-5.
- Open the pdf document above.
• O2k-Network Lab: AT_Innsbruck_Oroboros
Labels: MiParea: Respiration, Instruments;methods
HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-MultiSensor, MitoFitPublication, SUIT-013, SUIT-013 AmR ce D023, SUIT-009, SUIT-009 AmR mt D021, SUIT-009 AmR pce D019, SUIT-018, SUIT-018 AmR mt D031, SUIT-006 AmR mt D048, SUIT-003 AmR ce D017, SUIT-003 AmR ce D058, O2k-Analysis, DatLab
