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Revision as of 07:34, 18 July 2012 by Gnaiger Erich (talk | contribs) (Created page with " {{MitoPedia |abbr=MiR05Cr |description=Mitochondrial respiration medium, '''MiR05Cr''', developed for oxygraph incubations of mitochondrial preparations - ''[[permeabilized musc...")
Description
Mitochondrial respiration medium, MiR05Cr, developed for oxygraph incubations of mitochondrial preparations - permeabilized muscle fibres.
MiR05Cr = MiR05 + 20 mM creatine.
Abbreviation: MiR05Cr
Reference: MiPNet14.13
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
The oxygen solubility factor of MiR05Cr is the same as for MiR05 = 0.92.
Rationale: as for MiR05, but less ADP is needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect.