Difference between revisions of "MitoKit-CII/Malonate-nv"
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m (Zhang Feiyuan moved page NV161/MitoKit-CII to MitoKit-CII/Malonate-nv) |
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{{MitoPedia | {{MitoPedia | ||
|abbr=Mnanv | |abbr=Mnanv | ||
|description=''' | |description='''MitoKit-CII/Malonate-nv''' (diacetoxymethyl malonate) is a plasma membrane-permeable prodrug (permeable malonate; Mnanv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing [[malonate]] in the intracellular space. NeuroVive #: NV161 | ||
|info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]] | |info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]] | ||
}} | }} | ||
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== Application in HRR == | == Application in HRR == | ||
:::: ''' | :::: '''Malonate-nv:''' 10 mg, 7.7 µL, 5.24 M, MW=248.1 g.mol<sup>-1</sup> | ||
:::: '''Preparation of 100 mM stock solution:''' | :::: '''Preparation of 100 mM stock solution:''' | ||
::::# Add 395.78 µL DMSO in 7.7 µL NV161. | ::::# Add 395.78 µL DMSO in 7.7 µL NV161. | ||
| Line 15: | Line 15: | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
:::: '''Preparation of 10 mM stock solution (from the 100 mM stock):''' | :::: '''Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):''' | ||
::::# Dilute 40 µL 100 mM stock solution with 360 µL DMSO. | ::::# Dilute 40 µL 100 mM stock solution with 360 µL DMSO. | ||
::::# Divide into 100 µL portions. | ::::# Divide into 100 µL portions. | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
:::» '''O2k manual titrations''' | :::» '''O2k manual titrations''' | ||
::::* Event name: Mnanv | |||
::::* Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber). | ::::* Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber). | ||
::::* Final concentration: 5 µM steps. | ::::* Final concentration: 5 µM steps. | ||
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type. | ::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type. | ||
Revision as of 17:07, 3 June 2019
- high-resolution terminology - matching measurements at high-resolution
MitoKit-CII/Malonate-nv
Description
MitoKit-CII/Malonate-nv (diacetoxymethyl malonate) is a plasma membrane-permeable prodrug (permeable malonate; Mnanv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing malonate in the intracellular space. NeuroVive #: NV161
Abbreviation: Mnanv
Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
Application in HRR
- Malonate-nv: 10 mg, 7.7 µL, 5.24 M, MW=248.1 g.mol-1
- Preparation of 100 mM stock solution:
- Add 395.78 µL DMSO in 7.7 µL NV161.
- Divide into 40 µL portions.
- Store at -20 °C.
- Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):
- Dilute 40 µL 100 mM stock solution with 360 µL DMSO.
- Divide into 100 µL portions.
- Store at -20 °C.
- Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):
- » O2k manual titrations
- Event name: Mnanv
- Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 5 µM steps.
- Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
- » O2k manual titrations
