Difference between revisions of "MitoKit-CII MitoPedia"
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{{MitoPedia | {{MitoPedia | ||
|abbr=MitoKit-CII | |abbr=MitoKit-CII | ||
|description='''Cell permeable prodrugs''', composed of [[NV161]] and [[NV118]], of the Complex II substrate succinate increase ATP-linked mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres. | |description='''Cell permeable prodrugs''', composed of [[NV161/MitoKit-CII]] and [[NV118/MitoKit-CII]], of the Complex II substrate succinate increase ATP-linked mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres. | ||
|info=[[MitoKit-CII]], [[Ehinger 2016 Nat Commun]] | |info=[[MitoKit-CII]], [[Ehinger 2016 Nat Commun]] | ||
}} | }} | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
=== Preparation === Β | === Preparation === Β | ||
:::: Both compounds are dissolved in DMSO | :::: Both compounds are dissolved in DMSO and stored in aliquots at -20 Β°C. Β | ||
:::: We recommend the following dilutions for reaching the desired concentration in the experiments: | :::: We recommend the following dilutions for reaching the desired concentration in the experiments: | ||
::::* NV118: 100 mM stock solution | ::::* NV118/MitoKit-CII: 100 mM stock solution | ||
::::* NV161: 10 mM stock solution | ::::* NV161/MitoKit-CII: 10 mM stock solution | ||
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Revision as of 17:12, 28 March 2019
Description
Cell permeable prodrugs, composed of NV161/MitoKit-CII and NV118/MitoKit-CII, of the Complex II substrate succinate increase ATP-linked mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres.
Abbreviation: MitoKit-CII
Reference: MitoKit-CII, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
- For preparation, see MitoKit-CII.
Application in HRR
Preparation
- Both compounds are dissolved in DMSO and stored in aliquots at -20 Β°C.
- We recommend the following dilutions for reaching the desired concentration in the experiments:
- NV118/MitoKit-CII: 100 mM stock solution
- NV161/MitoKit-CII: 10 mM stock solution