Difference between revisions of "MitoOx1"

Latest revision as of 13:14, 12 February 2021

high-resolution terminology - matching measurements at high-resolution

MitoOx1

Mitochondrial respiration medium, MitoOx1, used by the Budapest groups for respirometry und Amplex Red trials.

Abbreviation: MitoOx1

Reference: Komary 2010 Biochim Biophys Acta

MitoPedia topics: Media for respirometry

MitoOx1: Mitochondrial Respiration Medium for measuring the H₂O₂ production

Oxygen solubility factor of MitoOx1 at 30 °C and 37 °C = 0.95

Compound	Final conc.	M [g·mol^-1]	Company, product code and storage
KCl	125 mM	74.55	Sigma 31248, store at RT
HEPES free acid	20 mM	238.31	Sigma H-7523 store at RT
KH₂PO₄	2 mM	136.09	Sigma P-5655, store at RT
MgCl₂	2.86 mM	203.30	Scharlau MA0036, store at RT
EGTA free acid	0.2 mM	380.35	Sigma E-4378, store at RT
BSA essentially fatty acid free	0.025%	-	Sigma A-6003, store at 2 - 8 °C

adjust pH to 7.0 (room temperature) with KOH

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+{{MitoPedia concepts|type=Chemicals
-|mitopedia method=Respirometry
+}}
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 '''MitoOx1: Mitochondrial Respiration Medium for measuring the H<sub>2</sub>O<sub>2</sub> production'''
-::Oxygen solubility factor of MitoOx1 at 30°C and 37°C = 0.95
+::Oxygen solubility factor of MitoOx1 at 30 °C and 37 °C = 0.95
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-! Compound !! Final conc. !! FW !! Company, product code and storage
+! Compound !! Final conc. !! M [g·mol<sup>-1</sup>] !! Company, product code and storage
 |-
 | KCl || 125 mM || 74.55 || align="right" |Sigma 31248, store at RT