Difference between revisions of "MitoOx1"

Revision as of 12:56, 23 July 2012

high-resolution terminology - matching measurements at high-resolution

MitoOx1

Mitochondrial respiration medium, MitoOx1 used by the Budapest groups for respirometry und Amplex Red trials.

Abbreviation: MitoOx2

MitoPedia methods: Respirometry

MitoPedia topics: Media for respirometry

MitoOx1: Mitochondrial Respiration Medium for measuring the H₂O₂ production

Oxygen solubility factor of MitoOx2 at 30°C and 37°C = 0.95

Compound	Final conc.	FW	Company, product code and storage
KCl	125 mM	74.55	Sigma 31248, store at RT
HEPES free acid	20 mM	238.31	Sigma H-7523 store at RT
KH₂PO₄	2 mM	136.09	Sigma P-5655, store at RT
MgCl₂	2.86 mM	203.30	Scharlau MA0036, store at RT
EGTA free acid	0.2 mM	380.35	Sigma E-4378, store at RT
BSA essentially fatty acid free	0.025%	-	Sigma A-6003, store at 2 - 8 °C

adjust pH to 7.0 (room temperature) with KOH

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 {{MitoPedia
 |abbr=MitoOx2
-|description='''Mitochondrial  respiration medium, MitoOx1''' used by the Budapest groups for respirometry und Amplex Red trials. MitoOx1 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without  H<sub>2</sub>O<sub>2</sub> present) for Amplex UltraRed(R) than e.g. [[MiR05|MiR05]].
+|description='''Mitochondrial  respiration medium, MitoOx1''' used by the Budapest groups for respirometry und Amplex Red trials.
 |type=Chemicals
 }}