Difference between revisions of "MitoOx1"
From Bioblast
| Line 2: | Line 2: | ||
|abbr=MitoOx1 | |abbr=MitoOx1 | ||
|description='''Mitochondrial respiration medium, MitoOx1''' used by the Budapest groups for respirometry und Amplex Red trials. | |description='''Mitochondrial respiration medium, MitoOx1''' used by the Budapest groups for respirometry und Amplex Red trials. | ||
|info=http://www.bioblast.at/index.php/Komary_2010_Biochim_Biophys_Acta | |||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
Revision as of 13:08, 23 July 2012
- high-resolution terminology - matching measurements at high-resolution
MitoOx1
Description
Mitochondrial respiration medium, MitoOx1 used by the Budapest groups for respirometry und Amplex Red trials.
Abbreviation: MitoOx1
Reference: http://www.bioblast.at/index.php/Komary_2010_Biochim_Biophys_Acta
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
Application in HRR
MitoOx1: Mitochondrial Respiration Medium for measuring the H2O2 production
- Oxygen solubility factor of MitoOx1 at 30°C and 37°C = 0.95
Chemicals for MitoOx2
| Compound | Final conc. | FW | Company, product code and storage |
|---|---|---|---|
| KCl | 125 mM | 74.55 | Sigma 31248, store at RT |
| HEPES free acid | 20 mM | 238.31 | Sigma H-7523 store at RT |
| KH2PO4 | 2 mM | 136.09 | Sigma P-5655, store at RT |
| MgCl2 | 2.86 mM | 203.30 | Scharlau MA0036, store at RT |
| EGTA free acid | 0.2 mM | 380.35 | Sigma E-4378, store at RT |
| BSA essentially fatty acid free | 0.025% | - | Sigma A-6003, store at 2 - 8 °C |
adjust pH to 7.0 (room temperature) with KOH
