Mitroshina 2017 Mod Technol Med: Difference between revisions
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Revision as of 10:53, 17 July 2017
Mitroshina ΠV, Abogessimengane BZ, Urazov ΠD, Hamraoui I, Mishchenko Π’Π, Astrakhanova Π’Π, Shchelchkova NΠ, Lapshin RD, Shishkina Π’V, Belousova II, Mukhina IV, Vedunova ΠV (2017) Adaptive role of glial cell line-derived neurotrophic factor in cerebral ischemia. Mod Technol Med 9:68-76. |
Β» Open Access
Mitroshina ΠV, Abogessimengane BZ, Urazov ΠD, Hamraoui I, Mishchenko Π’Π, Astrakhanova Π’Π, Shchelchkova NΠ, Lapshin RD, Shishkina Π’V, Belousova II, Mukhina IV, Vedunova ΠV (2017) Mod Technol Med
Abstract: The aim of the investigation was to study the effect of glial cell line-derived neurotrophic factor (GDNF) on animals` resistance to cerebral-ischemia-induced damage.
Materials and Methods. In vivo studies were carried out on C3H male mice weighing 18β40 g. Ischemia modeling was performed by bilateral irreversible occlusion of both carotid arteries. A neurological status as well as an orientative-exploratory behavior of experimental animals and their learning capability in the post-ischemic period were analyzed by using βOpen fieldβ and βPassive avoidanceβ tests. In addition, high-resolution respirometer Oxygraph-2k (Oroboros, Austria) was applied to study an oxygen uptake rate of brain mitochondria in ischemic conditions.
Results. GDNF application in bilateral occlusion of carotid arteries was found to contribute to the neurological status recovery. Moreover, it normalizes oxygen uptake rate of mitochondria in the post-ischemic period.
Conclusion. GDNF has a marked neuroprotective and antihypoxic effect under ischemia modeling in vivo. β’ Keywords: Glial cell line-derived neurotrophic factor, GDNF, Neuroprotection, Cerebral ischemia β’ Bioblast editor: Kandolf G β’ O2k-Network Lab: RU Nizhny Novgorod Mukhina I
Labels: MiParea: Respiration
Stress:Ischemia-reperfusion Organism: Mouse Tissue;cell: Nervous system Preparation: Isolated mitochondria
Coupling state: OXPHOS
Pathway: N, S
HRR: Oxygraph-2k
Labels, 2017-07