Myxothiazol: Difference between revisions

Revision as of 12:53, 22 November 2010

high-resolution terminology - matching measurements at high-resolution

Myxothiazol

Description

Inhibitor of [CIII] (inhibits also [CI]; G. Lenaz).

Abbreviation: Myx

Labels:

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

Application in HRR

Myx: Myxothiazol (Myxothiazol from Myxobacterium myxococcus fulvus, ~95%, HPLC), C₂₅H₃₃N₃O₃S₂); Sigma M 5779, 1 mg, store at 4 °C): FW = 487.7)

Caution: Very toxic. Chemical is currently not available 2010-11-22. Alternatively use Antimycin A as a Complex III inhibitor.

Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 1 mg of Myxothiazol directly into a 2 ml glass vial

2) add 2.05 ml Ethanol

3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution

4) store in glass vials (recommendation) at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

Titration volume: 1 µl using a 10 µl syringe
Final conc. in 2 ml O2k-chamber: 0.5 µM

@@ Line 6: / Line 6: @@
 }}
 {{Labeling
+|kinetics=Inhibitor; Uncoupler
 |instruments=Chemicals; Media
-|kinetics=Inhibitor; Uncoupler
+|type=Inhibitor ETS
 }}
 == Application in [[HRR]] ==
@@ Line 17: / Line 17: @@
-'''Preparation of 1 mM stock solution''' (dissolved in 99.9% Ethanol):
+'''Preparation of 5 mM stock solution''' (dissolved in 99.9% Ethanol):
-::1) weigh 491.2 mg of ADP (1 mmol ADP)
-::2) add 1.3 ml H<sub>2</sub>O  !White precipitate will occur, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature!
-::3) neutralize with 5 N KOH (approx. 465 µl)  !ADP will dissolve after addition of KOH!  (this is '''Solution A''')
-::4) check pH and adjust to 7 if necessary
-::5) adjust final volume to 2 ml and divide into 0.2 ml portions
-::6) store frozen at -80 °C
+::1) weigh 1 mg of Myxothiazol directly into a 2 ml glass vial
+::2) add 2.05 ml Ethanol
+::3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
+::4) store in glass vials (recommendation) at -20 °C
 '''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]