Neyroud 2019 J Muscle Res Cell Motil

Neyroud D, Nosacka RL, Judge AR, Hepple RT (2019) Colon 26 adenocarcinoma (C26)-induced cancer cachexia impairs skeletal muscle mitochondrial function and content. J Muscle Res Cell Motil [Epub ahead of print].

» PMID: 30945134

Neyroud D, Nosacka RL, Judge AR, Hepple RT (2019) J Muscle Res Cell Motil

Abstract: The present study aimed to determine the impact of colon 26 adenocarcinoma (C26)-induced cancer cachexia on skeletal muscle mitochondrial respiration and content. Twelve male CD2F1 mice were injected with C26-cells (tumor bearing (TB) group), whereas 12 age-matched mice received PBS vehicle injection (non-tumor bearing (N-TB) group). Mitochondrial respiration was studied in saponin-permeabilized soleus myofibers. TB mice showed lower body weight (~ 20%) as well as lower soleus, gastrocnemius-plantaris complex and tibialis anterior masses versus N-TB mice (p < 0.05). Soleus maximal state III mitochondrial respiration was 20% lower (10 mM glutamate, 5 mM malate, 5 mM adenosine diphosphate; p < 0.05) and acceptor control ratio (state III/state II) was 15% lower in the TB vs. N-TB (p < 0.05), with the latter suggesting uncoupling. Lower VDAC protein content suggested reduced mitochondrial content in TB versus N-TB (p < 0.05). Skeletal muscle in C26-induced cancer cachexia exhibits reductions in: maximal mitochondrial respiration capacity, mitochondrial coupling and mitochondrial content. • Keywords: Mitochondrial content, Mitochondrial coupling, Mitochondrial homeostasis, Mitochondrial respiration, Muscle wasting, Oxidative phosphorylation • Bioblast editor: Plangger M • O2k-Network Lab: US FL Gainesville Hepple RT

Labels: MiParea: Respiration  Pathology: Cancer 

Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized cells 

Coupling state: OXPHOS  Pathway: N, NS  HRR: Oxygraph-2k 

Labels, 2019-04