Nigericin: Difference between revisions
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|description=Nigericin | |description= Nigericin is a H+/K+ antiporter, which allows the electroneutral transport of these two ions in opposite directions across the mitochondrial inner membrane following the K+ concentration gradient. In the presence of K+, nigericin decreases pH in the mitchondrial matrix, thus, almost fully collapses the transmembrane pH gradient, which leads to the compensatory increase of [[Mitochondrial membrane potential|mt-membrane potential]]. Therefore, it is ideal to use to dissect the two components of the [[Protonmotive force|protonmotive force]], delta pH and [[Mitochondrial membrane potential|mt-membrane potential]]. It is recommended to use the lowest possible concentration of nigericin, which creates a maximal mitochondrial hyperpolarization. In the study of [[Komlodi 2018 J Bioenerg Biomembr]], 20 nM was applied on brain mitochondria isolated from guinea-pigs using 5 mM [[Succinate|succinate]] in the [[LEAK-respiration|LEAK state]] which cauesed maximum hyperpolarisation, but did not fully dissipate transmembrane pH gradient. Selivanov and his co-workers [https://www.ncbi.nlm.nih.gov/pubmed/18687689] and Lambert [https://www.ncbi.nlm.nih.gov/pubmed/15175007], however, used 100 nM nigericin, which in their hands fully collapsed transmembrane pH gradient using succinate as a respiratory substrate on isolated rat brain and skeletal muscle in the [[LEAK-respiration|LEAK state]]. | ||
|info=[[Dalmonte_2009_J_Biol_Chem]], [http://en.wikipedia.org/wiki/Nigericin Wikipedia] | |||
|info=[[Dalmonte_2009_J_Biol_Chem]], [[Komlodi 2018 J Bioenerg Biomembr]], [http://en.wikipedia.org/wiki/Nigericin Wikipedia] | |||
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{{MitoPedia concepts | {{MitoPedia concepts | ||
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{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry, Fluorometry | ||
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{{MitoPedia O2k and high-resolution respirometry}} | {{MitoPedia O2k and high-resolution respirometry}} | ||
{{MitoPedia topics}} | {{MitoPedia topics}} | ||
Revision as of 11:42, 5 July 2019
- high-resolution terminology - matching measurements at high-resolution
Nigericin
Description
Nigericin is a H+/K+ antiporter, which allows the electroneutral transport of these two ions in opposite directions across the mitochondrial inner membrane following the K+ concentration gradient. In the presence of K+, nigericin decreases pH in the mitchondrial matrix, thus, almost fully collapses the transmembrane pH gradient, which leads to the compensatory increase of mt-membrane potential. Therefore, it is ideal to use to dissect the two components of the protonmotive force, delta pH and mt-membrane potential. It is recommended to use the lowest possible concentration of nigericin, which creates a maximal mitochondrial hyperpolarization. In the study of Komlodi 2018 J Bioenerg Biomembr, 20 nM was applied on brain mitochondria isolated from guinea-pigs using 5 mM succinate in the LEAK state which cauesed maximum hyperpolarisation, but did not fully dissipate transmembrane pH gradient. Selivanov and his co-workers [1] and Lambert [2], however, used 100 nM nigericin, which in their hands fully collapsed transmembrane pH gradient using succinate as a respiratory substrate on isolated rat brain and skeletal muscle in the LEAK state.
Reference: Dalmonte_2009_J_Biol_Chem, Komlodi 2018 J Bioenerg Biomembr, Wikipedia
MitoPedia concepts:
Respiratory state
MitoPedia methods:
Respirometry,
Fluorometry
