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O2k-Virtual Support |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
O2k-Virtual support includes 8 individual hours. Via a live video link, Oroboros experts guide you step-by-step on topics of your choice, such as O2k instrumental setup and service of the polarographic oxygen sensors (POS) for instrumental quality control, an essential component of HRR. This offers the opportunity to analyze and discuss your experimental DatLab files obtained with your O2k with the bioenergetics experts of Oroboros. It offers flexibility to participants and gives the option to choose virtual sessions that best fit individual needs.
O2k-Virtual support hour allocation
- Individual hours can be used for any topics needed, such as to prepare experiments, answer any other related questions, etc. The timetable in the program shows an approximate estimation of how much time will be needed for each specific session. This overview is intended to help participants plan their available 8 hours efficiently.
- The duration is estimated for participants who still do not have experience with the O2k, sessions might take less time for experienced O2k users.
- More than one topic can be covered in a single session and the minimum time per session is 1 hour.
- Sessions with "Do-it-yourself" hours do not count toward the 8 hours with the tutor.
- The O2k-Virtual support sessions will take place on the dates confirmed by the participants and tutor.
Materials for self-study
- It is highly recommended that participants prepare for their sessions by going through the self-study material.
- » O2k-Virtual Support self-study material
Program
- Participants can use the below sample program as a guideline for possible sessions.
- For beginners we recommend starting with the Installation and startup support session session (Part 1 in the program). Experienced users may choose to select sessions from both the O2k-Basic and Advanced programs.
O2k-Basic
| Session | Duration |
|---|---|
| Part 1: Installation and startup support session | |
| Part 1.1: POS service and O2k instrumental setup | |
Hands-on: Installation of the O2k
|
2.5 h |
| Part 1.2: DatLab 8 overview and O2 calibration | |
| DatLab overview Hands-on: Quality control 1: Oxygen calibration
|
1.5 h |
| Part 1.3: O2 background | |
Hands-on: Quality control 2: Oxygen background
|
Do-it-yourself 2 h |
| DatLab analysis and discussion | Start-up 1 h |
| Part 2 | |
| Part 2.1: Biological samples and experimental design | |
Discussion about biological samples, experimental design, SUIT protocols
|
1-2 h |
| Part 2.2: Biological experiment and data analysis | |
Hands-on: Quality control 1: Oxygen calibration
|
Do-it-yourself 1.5 h |
Hands-on: Biological experiment: cell or mitochondrial respiration
|
Do-it-yourself 1-3 h |
Hands-on: O2k-cleaning after use
|
Do-it-yourself 1 h |
| Hands-on: DatLab analysis and DatLab performance evaluation. Discussion |
1-2 h |
O2k-Advanced: Simultaneous determination of O2 and H2O2 fluxes
- Some of the hours from the basic program can be shortened or replaced by advanced sessions:
| Session | Duration
|
|---|---|
Introduction to H2O2 measurements and discussion
|
1 h |
Hands-on: Quality control 1: Oxygen calibration
|
Do-it-yourself 1.5 h
|
Hands-on: Amplex UltraRed calibration: Amplex UltraRed calibration in the absence of biological sample.
|
1 h |
Hands-on: Biological experiment: simultaneous measurement of O2 and H2O2 production
|
Do-it-yourself2 h |
Hands-on: O2k-cleaning after use
|
Do-it-yourself1 h |
| Hands-on: DatLab H2O2 analysis and DatLab performance evaluation. Discussion |
1.5 h |
Contact
- Oroboros Instruments
- High-Resolution Respirometry
- Schoepfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and Cell Research
