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| {{OROBOROS_header_page_name}}
| | #REDIRECT [[Oroboros O2k]] |
| :::: <big>'''[[Oxygraph-2k |OROBOROS Oxygraph-2k]] - unique for high-resolution respirometry (HRR)'''</big>
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| ::::::::::: <big>'''O2k - tested and trusted'''</big>
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| [[Image:Oxygraph-2k-Concept.jpg|right|300px|link=O2k high-resolution respirometry |HRR]]
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| == The O2k for high-resolution respirometry ==
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| [[Image:O2k-Fluorometer.JPG|left|120px|link=http://wiki.oroboros.at/index.php/Oxygraph-2k |O2k]]
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| The OROBOROS Oxygraph-2k provides a unique high-resolution approach for the monitoring of cellular and mitochondrial respiratory function: [[high-resolution respirometry |high-resolution respirometry (HRR)]]. Considering mitochondria as the powerhouses of the cell, HRR introduced the concept of [[cell ergometry]]. Are your cells [[MitoFit]]? HRR is now applied world-wide for the analysis of mitochondrial and metabolic diseases, in the rapidly growing field of ischemia-reperfusion injury, oxidative stress, apoptosis, aging); biopsies with limited amounts of sample (diagnosis of genetic and acquired mitochondrial defects in pediatric patients, diabetes); cell cultures with limited cell numbers or low activities; mutants with diminished respiratory capacity; chemical oxidation rates and antioxidant capacities; and respiratory measurements at low, physiological intracellular oxygen levels and oxygen kinetics.
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| [[File:OROBOROS MitoFit-Lab.jpg|left|240px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |OROBOROS]]
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| [[File:OROBOROS-Concept.jpg|right|300px|link=OROBOROS Company]]
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| High-resolution respirometry by the OROBOROS O2k is based on a radically different systems approach to polarographic measurement of oxygen dynamics. High-resolution respirometry rests on several tightly connected instrumental features and concepts integrated by the DatLab software, providing on-line display of oxygen concentration and respiration ([[oxygen flux]]). The mechanics, critical selection of materials, and state-of-the-art electronics provide the basis of signal stability of an optimized [[polarographic oxygen sensor]], in an electronically controlled thermal environment with high temperature stability. Calibrations and instrumental controls follow standardized procedures, with highly automatic on-line analysis by the DatLab software. Scientific support is complementary to the approach of high-resolution respirometry.
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| [[Image:The world as a laboratory.jpg|left|120px|link=http://wiki.oroboros.at/index.php/The_world_as_a_laboratory |The world as a laboratory]]
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| While the OROBOROS O2k provides the instrumental basis for high-resolution respirometry, successful operation at high accuracy up to the limit of detection depends on a professional application by the technician, scientist or student. Participation in a hands-on introductory [[O2k-Workshop]] is recommended to save time and gain from the experience obtained over many years of application in various [[The world as a laboratory |settings]].
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| == From the O2k to the Power-O2k ==
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| [[Image:Power-O2k.png|left|120px|link=http://wiki.oroboros.at/index.php/Power-O2k|Power-O2k]]
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| Multiple O2k-units are offered as a ‘Power-O2k System’ for high-resolution respirometry combined with high-throughput - at high output.
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| :: ''More details? Consult'' [[Power-O2k]]
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