Oligomycin

high-resolution terminology - matching measurements at high-resolution

Oligomycin

Description

Oligomycin is an inhibitor of ATP synthase.

Abbreviation: Omy

Reference: MiPNet09.12

MitoPedia topics: Inhibitor 

Labels:

Enzyme: Complex V; ATP Synthase"Complex V; ATP Synthase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. 

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Application in HRR

Omy: Oligomycin (Oligomycin from Streptomyces diastatochromogenes, mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg, store at -20 °C: FW = 800

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 4 mg/ml (5 mM) stock solution (dissolved in 99.9% Ethanol):

1) weigh 4 mg of Oligomycin into a 2 ml glass vial and dissolve in 1 ml Ethanol

2) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution

3) store in glass vials (recommendation) at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

• Titration volume: 1 µl using a 10 µl syringe
• Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM)