Oligomycin: Difference between revisions

Revision as of 14:05, 11 November 2013

high-resolution terminology - matching measurements at high-resolution

Oligomycin

Description

Oligomycin is an inhibitor of ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production). The inhibition of ATP synthesis would also stop the electron transport chain. In oxidative phosphorylation research, it is used to prevent state 3 (phosphorylating) respiration.

Abbreviation: Omy

Reference: MiPNet09.12

MitoPedia topics: Inhibitor

Application in HRR

Omy: Oligomycin (Oligomycin from Streptomyces diastatochromogenes, mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg, store at -20 °C; FW = 800

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 4 mg of Oligomycin into a 2 ml glass vial

2) dissolve in 1 ml Ethanol

3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution

4) store in glass vials (recommendation) at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

Titration volume: 1 µl using a 10 µl syringe
Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM)

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Omy
-|description='''Oligomycin''' (Omy) is an inhibitor of [[ATP synthase]] by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production). The inhibition of ATP synthesis also inhibits respiration. In OXPHOS analysis, Omy is used to induce a [[LEAK]] state of respiration.
+|description='''Oligomycin''' is an inhibitor of [[ATP synthase]] by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production). The inhibition of ATP synthesis would also stop the electron transport chain. In oxidative phosphorylation research, it is used to prevent [[State 3|state 3]] (phosphorylating) respiration.
 |info=[[MiPNet09.12]]
 |type=Inhibitor PS
-}}
-{{MitoPedia methods|type=Inhibitor PS
 }}
 {{MitoPedia topics
-|mitopedia topic=Respiratory state, Inhibitor
+|mitopedia topic=Inhibitor
 |type=Inhibitor PS
 }}
 == Application in [[HRR]] ==
-'''Omy: Oligomycin''' (Oligomycin from ''Streptomyces diastatochromogenes''; mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg; store at -20 °C; FW = 800.
+'''Omy: Oligomycin''' (Oligomycin from ''Streptomyces diastatochromogenes'', mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg, store at -20 °C; FW = 800
-'''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
+<span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
-'''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol):
-::1) Weigh 4 mg of Oligomycin into a 2 ml glass vial.
+'''Preparation of 5 mM stock solution''' (dissolved in 99.9% Ethanol):
-::2) Dissolve in 1 ml ethanol.
-::3) Divide into 0.2 ml portions or store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the storage solution.
-::4) Store in glass vials (recommendation) at -20 °C.
+::1) weigh 4 mg of Oligomycin into a 2 ml glass vial
+::2) dissolve in 1 ml Ethanol
+::3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
+::4) store in glass vials (recommendation) at -20 °C
-'''Oxygraph-2k manual titrations:'''  [[MiPNet09.12]]
-::* Titration volume: 1 µl using a 10 µl syringe.
+'''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
-::* Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM).
- [[Talk:Oligomycin|>>Discussion]]
+::* Titration volume: 1 µl using a 10 µl syringe
+::* Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM)