Ouabain: Difference between revisions

Revision as of 10:31, 11 September 2020

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Ouabain

Description

Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.

Abbreviation: Oua

Reference: MiPNet03.02

Application in HRR

Oua: Ouabain octahydrate (≥95% (HPLC), powder; C₂₉H₄₄O₁₂_*8H₂0); Sigma O 3125, store at R.T.; FW = 728.77.

Caution: Light sensitive and toxic!

Preparation of 10 mM stock solution (dissolved in H₂O):

Weigh 7.3 mg ouabain.
Dissolve in 1 mL H₂O.
Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
Store in glass vials (recommendation) at -20 °C.

MitoPedia topics: Inhibitor

@@ Line 4: / Line 4: @@
 |info=[[MiPNet03.02]]
 }}
-{{MitoPedia methods}}
+__TOC__
-{{MitoPedia topics
-|mitopedia topic=Inhibitor
-}}
 == Application in [[HRR]] ==
-'''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; FW = 728.77.
+:::'''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; FW = 728.77.
 <span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span>
+::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
-'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
+::::# Weigh 7.3 mg ouabain.
+::::# Dissolve in 1 mL H<sub>2</sub>O.
-::1) Weigh 7.3 mg ouabain.
+::::# Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
-::2) Dissolve in 1 mL H<sub>2</sub>O.
+::::# Store in glass vials (recommendation) at -20 °C.
-::3) Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
+{{MitoPedia methods}}
-::4) Store in glass vials (recommendation) at -20 °C.
+{{MitoPedia topics
+|mitopedia topic=Inhibitor
+}}