From Bioblast
Oxygen sensor test |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
The O2 sensor test is described as an OroboPOS standard operating procedure: Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Β»MiPNet06.03 POS-Calibration-SOPΒ«
When should an O2-sensor test be performed?
- After switching on the O2k, every day: air calibration and stirrer test.
- Zero oxygen calibration: from time to time over weeks; bracketing zero oxygen calibrations when working at low oxygen.
- After application of a new membrane and O2-sensor service: In some cases, the signal of the OroboPOS improves (higher signal stability, less noise, shorter response time), when the O2k remains switched on over night (O2k-Chambers filled with 70% EtOH).
- During troubleshooting, particularly when switching components between the two chambers, a quick sensor test is performed after each step (stirrer test, sensor signal).
The main criteria for sensor performance
- Raw signal at air saturation
- Raw signal at zero oxygen
- Signal stability: drift and noise
- Response time of the POS
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