Oxygen signal: Difference between revisions
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|description=The '''oxygen signal''' of the [[O2k]] is transmitted from the electrochemical polarographic oxygen sensor ([[OroboPOS]]) for each of the two chambers to [[DatLab]]. The primary signal is a current [[mAmp]] which is converted into a voltage [V], and calibrated in SI units for amount of substrance concentration [ยตmol.dm-3 or ยตM]. ย | |description=The '''oxygen signal''' of the [[O2k]] is transmitted from the electrochemical polarographic oxygen sensor ([[OroboPOS]]) for each of the two chambers to [[DatLab]]. The primary signal is a current [[mAmp]] which is converted into a voltage [V], and calibrated in SI units for amount of substrance concentration [ยตmol.dm-3 or ยตM]. | ||
|info=[[MiPNet19.18D O2k-Calibration]], [[MiPNet06.03 POS-Calibration-SOP]] | |info=[[MiPNet19.18D O2k-Calibration]], [[MiPNet06.03 POS-Calibration-SOP]] | ||
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{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
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{{MitoPedia O2k and high-resolution respirometry | |||
|mitopedia O2k and high-resolution respirometry=DatLab | |||
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{{MitoPedia SUIT}} | {{MitoPedia SUIT}} | ||
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{{Template:Technical support integrated}} | {{Template:Technical support integrated}} | ||
=== Stability of the oxygen signal === | === Stability of the oxygen signal === |
Revision as of 10:54, 8 February 2016
Description
The oxygen signal of the O2k is transmitted from the electrochemical polarographic oxygen sensor (OroboPOS) for each of the two chambers to DatLab. The primary signal is a current mAmp which is converted into a voltage [V], and calibrated in SI units for amount of substrance concentration [ยตmol.dm-3 or ยตM].
Reference: MiPNet19.18D O2k-Calibration, MiPNet06.03 POS-Calibration-SOP
MitoPedia methods: Respirometry
MitoPedia O2k and high-resolution respirometry:
DatLab
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Stability of the oxygen signal
The stability of the oxygen signal of the O2k is evaluated by the (negative) uncorrected slope over time. After smoothing, the slope of a stable sensor should be less than +- 1 pmol.s-1.ml-1 in a 2-ml chamber.
- Instability of the oxygen signal
- There is drift of the oxygen signal over short or long periods of time.
- The signal at air saturation is abnormally high for a given gain setting.
- The zero current is high (>2.5%) and may show drift.
- Follow the standard instructions for oxygen sensor service.
Oxygen signal does not respond
- The oxygen signal remains at or close to zero even at high oxygen levels.
- The signal at air saturation is abnormally low.
- Check the gain setting for the affected oxygen channel. Set to Gain 1.
- Apply technical service: O2k-technical support and open innovation